Intracellular behavior of beryllium oxide particles: an in vitro study in murine macrophages.

Details

• Personal Author:
  Day, Gregory A. ; Dickerson RM ; Esman NA ; Hoover, Mark D. ; Peterson EJ ; Scripsick RC ; Stefaniak, Aleksandr B.
• Description:
  Beryllium metal, its oxide (BeO), and alloys are materials of industrial significance with recognized adverse effects on worker health. Currently, the degree of risk associated with exposure to these materials in the workplace is assessed through measurement of beryllium aerosol mass concentration, the control of which has proven ineffective at eliminating the occurrence of chronic beryllium disease. The rationale for this research was to examine the role of bioavailability, rather than mass alone, as a key factor for risk. Physicochemical changes were evaluated in vitro for well-characterized high-purity BeO particles engulfed by and retained witl2in murine J774A.1 monocyte-macrophage cells. The BeO was a commercially available powder consisting of diffuse clusters of 200-nm diameter primary particles. Following incubation for 124 to 144 hours, engulfed particles were recovered for re-characterization. Recovered particles were similar in morphology, chemical composition, and size to the original material, confirming the insoluble nature of the BeO particles. Measurable levels of dissolved beryllium, representing 0.3 to 4.8% of the estimated total beryllium mass added, were found in the recovered intracellular fluid; dissolved beryllium was not found in the extracellular fluid. Release of beryllium may occur in tl2e alveolar environment after macrophage death, in the lymphatic system after transport of beryllium by macrophages, or in the alveolar interstitium after migration and slower dissolution of beryllium particles in tissue. These findings demonstrate the bioavailability of dissolved beryllium, and are consistent with previously observed results in canine alveolar macrophages [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Beryllium Copper Dust Irritants Lung Lung Diseases Metals Metal Workers Occupational Health Particulate Matter Poisoning Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Beryllium-disease; Beryllium-poisoning; Beryllium-compounds; Metal-oxides; Metal-poisoning; Copper-alloys; Aerosols; Aerosol-particles; Airborne-particles; Airborne-dusts; Metal-compounds; Metal-dusts; Metal-fumes; Metal-industry; Metal-industry-workers; Metal-workers; Metallic-compounds; Metallic-dusts; Metallic-fumes; Metallic-poisoning; Metallic-poisons; Metals; Pulmonary-system-disorders; Pulmonary-disorders; Respirable-dust; Respiratory-irritants; Respiratory-system-disorders
• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Proceedings ; Abstract
• Place as Subject:
  Maryland ; New Mexico ; Ohio ; Oklahoma ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  169
• Issue:
  7
• NIOSHTIC Number:
  nn:20025897
• Citation:
  Am J Respir Crit Care Med 2004 Apr; 169(7)(Abstracts):A640
• Email:
  gday@cdc.gov
• CAS Registry Number:
  Beryllium (CAS RN 7440-41-7) ; Copper (CAS RN 7440-50-8)
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Performing Organization:
  Johns Hopkins University, Baltimore, Maryland
• Peer Reviewed:
  False
• Start Date:
  20010601
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Supplement:
  Abstracts
• End Date:
  20040531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247974/cdc_247974_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]