U.S. flag An official website of the United States government.
Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

i

Studies on biochemical determinants of quinone-induced toxicity in primary murine bone marrow stromal cells.



Details

  • Personal Author:
  • Description:
    Susceptibility to quinone (106514) generating metabolites of benzene (71432) and quinone-reductase (QR) activity in cultured bone marrow stromal cells derived from male DBA/2-mice was investigated. In order to identify underlying mechanisms of 1,2-dithiole-3-thione (DTT) chemoprotection, the effect of DTT on QR and glutathione-S- transferase (GST) activities in the stromal cells was assessed. DTT induced QR activity in primary stroma cells in a dose dependent manner. There were no corresponding changes in GST activity, indicating that induction of QR activity may be important in DTT's chemoprotection. Basal QR activity and percent esterase remained essentially constant in the beginning 2 to 3 weeks of primary culture of bone marrow stroma. The macrophages appeared to be more sensitive to HQ induced toxicity than the fibroblastic element of bone marrow stroma as indicated by treatment with HQ which increased QR activity, but reduced the percent esterase positive cells. DTT pretreatment 24 hours prior to administering HQ restored the percent esterase positive cells to control levels. In-vivo feeding studies with DTT induced QR activity within the bone marrow compartment. The authors conclude that QR activity and its inducibility may be important factors in chemically induced bone marrow toxicity and carcinogenicity. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISBN:
    9780306437373
  • ISSN:
    0065-2598
  • Publisher:
    Plenum Press
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Proceedings
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Volume:
    283
  • NIOSHTIC Number:
    nn:00213955
  • Citation:
    Adv Exp Med Biol, Biological Reactive Intermediates IV: Molecular and Cellular Effect and Their Impact on Human Health, Proceedings of the Fourth International Symposium on Biological Reactive Intermediates, January 14-17, 1990, Tucson, Arizona. Witmer CM, ed., NY: Plenum Press, 1991 Feb; 283:843-846
  • Contact Point Address:
    Environmental Health Sciences Johns Hopkins University 615 North Wolfe Street Baltimore, MD 21205
  • CAS Registry Number:
  • Editor(s):
    Witmer CM
  • Federal Fiscal Year:
    1991
  • Performing Organization:
    Johns Hopkins University, Baltimore, Maryland
  • Peer Reviewed:
    True
  • Start Date:
    19880930
  • Source Full Name:
    Advances in Experimental Medicine and Biology. Biological Reactive Intermediates IV: Molecular and Cellular Effect and Their Impact on Human Health, Proceedings of the Fourth International Symposium on Biological Reactive Intermediates, January 14-17, 1990, Tucson, Arizona
  • End Date:
    19900929
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
  • Download URL:
  • File Type:
    Filetype[PDF - 168.02 KB ]
PREVIOUS
ON THIS PAGE
Details
CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
BACK TO TOP