Transplacental genotoxicity of triethylenemelamine, benzene, and vinblastine in mice.

Details

• Personal Author:
  Chen K ; Ong T ; Shi X ; Wallace W ; Whong Z ; Wu L ; Xing SG
• Description:
  The genotoxicity of triethylenemelamine (51183) (TEM), benzene (71432), and vinblastine (865214) was investigated in transplacentally exposed mice. Groups of four female CD-1-mice were administered each test chemical by intraperitoneal injection on gestation day 14, and a second injection was given 24 hours (hr) later. Dose rates were 0.125, 0.25, and 0.5mg/kg of TEM, 439, 878, and 1318mg/kg of benzene, and 0.5, 1.0, and 2.0mg/kg of vinblastine. Animals were sacrificed at 40hr (for TEM and benzene), or 30hr (for vinblastine) after the first injection, and maternal bone marrow and fetal liver were sampled randomly for sister chromatid exchanges (SCE) and micronuclei (MN) formation as genetic endpoints. Results showed that TEM caused significant increases in MN and SCE of both erythrocytic precursor cells of the maternal bone marrow and fetal liver cells. Benzene produced the same results only at the highest concentration used, although even at this concentration, genotoxicity was lower than that of TEM. Benzene induced MN frequency was higher in fetal liver than in the maternal bone marrow. Vinblastine toxicity resulted in MN induction at a level seven times higher in maternal bone marrow than in fetal liver. SCE were absent with vinblastine treatment. The authors conclude that all three chemicals act as transplacental genotoxins and cause cytogenetic changes in bone marrow cells, with TEM being the most potent and benzene the weakest among them, and that maternal sensitivities are different to fetal sensitivities to these compounds. [Description provided by NIOSH]
• Subjects:
  Blood Carcinogens Chromosomes Cytotoxins DNA Fetus Genetics Laboratories Liver Mutagens Occupational Health Safety Teratogenesis Toxicology Urogenital System

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• Keywords:
  Author Keywords: SCE; Micronucleus; Benzene; Triethylenemelamine; Vinblastine NIOSH-Author; Blood-cells; Carcinogens; Chromosome-damage; Comparative-toxicology; Cytotoxic-effects; DNA-damage; Fetus; Genetic-factors; Hepatotoxicity; Laboratory-animals; Mutagens; Teratogens; Transplacental-exposure;
• ISSN:
  0270-3211
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  223-230
• Volume:
  12
• Issue:
  5
• NIOSHTIC Number:
  nn:00213562
• Citation:
  Teratog, Carcinog, Mutagen 1992 Jan; 12(5):223-230
• Contact Point Address:
  W.-Z. Whong, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505
• CAS Registry Number:
  Benzene (CAS RN 71-43-2) ; Triethylenemelamine (CAS RN 51-18-3) ; Vinblastine (CAS RN 865-21-4)
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  True
• Source Full Name:
  Teratogenesis, Carcinogenesis, and Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247790/cdc_247790_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]