Comparative in vivo and in vitro sister chromatid exchange studies in Chinese hamster bone marrow and spleen cells.

Details

• Personal Author:
  Krishna G ; Nath J ; Ong T
• Description:
  Bone marrow and spleen cells from Chinese hamsters were chosen as models for in-vivo and in-vivo/in-vitro cyclophosphamide (50180) induced sister chromatid exchange (SCE) comparison. Cyclophosphamide was dissolved in phosphate buffered saline and immediately injected intraperitoneally at doses of 10 through 40mg/kg. Cyclophosphamide caused dose related increases in SCEs in the cells under both in-vivo and in-vivo/in-vitro conditions. While the sensitivity of bone marrow and spleen cells to the effects of cyclophosphamide was similar in-vivo, it varied in in-vivo/in-vitro and was higher in spleen cells. Whether or not in-vivo/in-vitro studies can be used to predict in-vivo damage remains to be established by additional studies. Trinitrofluorenone (25322149), a direct acting mutagen, caused equivalent dose related increases in SCEs in both bone marrow and spleen cells in-vitro. These cell types, therefore, can be used as genotoxicity assays under both in- vivo/in-vitro and in-vitro conditions. These systems can be potentially useful in comparative in-vivo and in-vitro cytogenetic studies with same tissues to better assess the genotoxic risk of chemicals. [Description provided by NIOSH]
• Subjects:
  Amides Antineoplastic Agents Cells, Cultured Chromosomes Laboratories Occupational Health Safety
• Keywords:
  Author Keywords: Chinese Hamsters; Sister Chromatid Exchanges; Primary Cell Culture; Bone Marrow; Spleen; Cyclophosphamide; Trinitrofluorenone NIOSH-Author; Laboratory-animals; Chromosome-damage; Chromosome-disorders; Cell-cultures; Cell-metabolism; Antineoplastic-agents; Spleen-disorders; Amides;
• ISSN:
  0270-3211
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  321-330
• Volume:
  6
• Issue:
  4
• NIOSHTIC Number:
  nn:00163949
• Citation:
  Teratog, Carcinog, Mutagen 1986 Jan; 6(4):321-330
• CAS Registry Number:
  9H-Fluoren-9-one, trinitro- (CAS RN 25322-14-9) ; Cyclophosphamide (CAS RN 50-18-0)
• Federal Fiscal Year:
  1986
• Peer Reviewed:
  True
• Source Full Name:
  Teratogenesis, Carcinogenesis, and Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247573/cdc_247573_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]