Topological Organization of Multi-chromosomal Regions by Firre

Details

• Alternative Title:
  Nat Struct Mol Biol
• Personal Author:
  Hacisuleyman, Ezgi ; Goff, Loyal A. ; Trapnell, Cole ; Williams, Adam ; Henao-Mejia, Jorge ; Sun, Lei ; McClanahan, Patrick ; Hendrickson, David G. ; Sauvageau, Martin ; Kelley, David R. ; Morse, Michael ; Engreitz, Jesse ; Lander, Eric S. ; Guttman, Mitch ; Lodish, Harvey F. ; Flavell, Richard ; Raj, Arjun ; Rinn, John L.
• Description:
  RNA, including long noncoding RNA (lncRNA), is known to be an abundant and important structural component of the nuclear matrix. However, the molecular identities, functional roles and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we describe one lncRNA, Firre, that interacts with the nuclear-matrix factor hnRNPU through a 156-bp repeating sequence and localizes across an ~5-Mb domain on the X chromosome. We further observed Firre localization across five distinct trans-chromosomal loci, which reside in spatial proximity to the Firre genomic locus on the X chromosome. Both genetic deletion of the Firre locus and knockdown of hnRNPU resulted in loss of colocalization of these trans-chromosomal interacting loci. Thus, our data suggest a model in which lncRNAs such as Firre can interface with and modulate nuclear architecture across chromosomes.
• Keywords:
  Animals Base Sequence Chromatin Chromosomes Embryonic Stem Cells Female Humans Male Mice Models, Genetic Molecular Sequence Data RNA, Long Noncoding Sequence Analysis, RNA X Chromosome Inactivation

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• Source:
  Nat Struct Mol Biol. 21(2):198-206
• Pubmed ID:
  24463464
• Pubmed Central ID:
  PMC3950333
• Document Type:
  Journal Article
• Funding:
  1DP20D008514/DP/NCCDPHP CDC HHSUnited States/ ; DP2 OD006670/OD/NIH HHSUnited States/ ; P01 GM099117/GM/NIGMS NIH HHSUnited States/ ; 1DP2OD00667/OD/NIH HHSUnited States/ ; HHMI/Howard Hughes Medical InstituteUnited States/ ; P01GM099117/GM/NIGMS NIH HHSUnited States/ ; R01 DK068348/DK/NIDDK NIH HHSUnited States/ ; P50 HG006193/HG/NHGRI NIH HHSUnited States/ ; R01 DK047618/DK/NIDDK NIH HHSUnited States/ ; DP2 OD008514/OD/NIH HHSUnited States/ ; P50HG006193-01/HG/NHGRI NIH HHSUnited States/
• Volume:
  21
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:757153c6e3f9bb090087728af73273bc1bafa2378842bc7a12388e56407937db
• Download URL:
  https://stacks.cdc.gov/view/cdc/24458/cdc_24458_DS1.pdf
• File Type:
  [PDF - 2.11 MB ]