Chlorophyllin: an antigenotoxic agent.

Details

• Personal Author:
  Brockman HE ; Ong T ; Whong Z
• Description:
  The antimutagenic and antitransforming activities of chlorophyllin were investigated and compared with the antimutagenic activities of vitamin-C vitamin-E, retinol and beta-carotene. In the Ames Salmonella reverse mutation test, a concentration of 1.25 milligrams (mg) chlorophyllin per plate inhibited the mutagenicity of airborne particles (AP), cigarette smoke (CS), fried shredded pork (FSP) and fried beef (FB). Inhibitions of 92% to 98% by chlorophyllin of the mutagenicity of diesel emission particles (DEP), coal dust (CD), red grape juice (RGJ), and red wine (RW) were observed. Inhibitions of 75% to 80% by concentrations of 10mg chlorophyllin per plate were seen in the mutagenicity of tobacco snuff (TS) and chewing tobacco (CT). Concentrations of 2.5mg chlorophyllin per plate achieved inhibitions of 60% to 100% in mutagenicity of AP, CD, DEP, TS, and BP in the forward mutagen assay. Inhibition of DEP mutagenicity was not decreased by heating chlorophyllin for 10 minutes at 100 degrees- C. Reconstruction assays demonstrated that the observed inhibition of mutagenicity of test mixtures by chlorophyllin was not the result of inhibition of revertant cell divisions. At equal concentrations, retinol exhibited 50% of the inhibitory effect of chlorophyllin of the complex mixtures tested. Vitamin-E and beta-carotene inhibited less than 20% of the mutagenicity of the test mixtures, with the exception of AP, which beta-carotene inhibited by 39%. Vitamin-C inhibited none of the mutagenicities, increasing the mutagenic effect of AP by 60%. At a concentration of 150 micrograms/milliliter, chlorophyllin inhibited the transforming activities of benzo(a)pyrene (50328), N-methyl-N'-nitro-N- nitroguanidine (70257), 3-methylcholanthrene (56495), aflatoxin-B1 (1162658), 7,12-dimethylbenz(a)anthracene (57976), CD, CT and TS from 61% to 99%. The authors conclude that chlorophyllin is an antigenotoxic agent, effectively inhibiting the mutagenicity of the test mixtures. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Alcoholic Beverages Coal Dust Hydrocarbons Mutagens Occupational Health Particulate Matter Polycyclic Aromatic Hydrocarbons Safety Smoke Tobacco Products Vehicle Emissions

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• Keywords:
  Mutagens; Airborne-particles; Coal-dust; Diesel-emissions; Tobacco-smoke; Alcoholic-beverages; Vitamins; Polycyclic-aromatic-hydrocarbons; Microbial-test-systems
• ISBN:
  9780841227682
• Publisher:
  American Chemical Society Symposium Series
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  272-281
• Volume:
  1
• NIOSHTIC Number:
  nn:00232439
• Citation:
  Food Phytochemicals for Cancer Prevention, I. Fruits and Vegetables. Huang M-T, ed. Washington, DC: American Chemical Society, ACS Symposium Series 546, 1994 Jan; 1:272-281
• CAS Registry Number:
  3-Methylcholanthrene (CAS RN 56-49-5) ; 7,12-Dimethylbenz[a]anthracene (CAS RN 57-97-6) ; Aflatoxin B1 (CAS RN 1162-65-8) ; Benzo[a]pyrene (CAS RN 50-32-8) ; N-Methyl-N′-nitro-N-nitrosoguanidine (CAS RN 70-25-7)
• Editor(s):
  Huang MT
• Federal Fiscal Year:
  1994
• Peer Reviewed:
  False
• Source Full Name:
  Food Phytochemicals for Cancer Prevention, I. Fruits and Vegetables
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/243472/cdc_243472_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]