Metabolism And Distribution Of Two 14C-Benzidine-Congener-Based Dyes In Rats As Determined By GC, HPLC, And Radioassays.

Details

• Personal Author:
  Billedeau SM ; Bowman MC ; Lowry, Larry K. ; Nony CR ; Oller WL ; Rowland KL
• Description:
  The metabolism and distribution of Direct-Red-2 (992596) (DR) and Direct-Blue-15 (2429745) (DB) were studied in rats. Male Fischer- 344-rats were administered 12 milligrams per kilogram carbon-14 (C- 14) labeled DR or DB or molar equivalent doses of the corresponding amines, dimethylbenzidine (119937) or dimethoxybenzidine (1331539), respectively. Urine and feces samples were collected for up to 192 hours after dosing and assayed for C-14 activity and metabolites. Selected animals were killed 2 to 72 hours after administration and the tissue distribution of C-14 activity was determined. Most of the C-14 activity was excreted in the feces, 52.05 to 74.40 percent of the dose being eliminated. Urinary excretion amounted to 18.79 to 39.66 percent of the dose. Dimethylbenzidine and dimethoxybenzidine were more extensively metabolized than the corresponding dyes, DR and DB. Urinary metabolites produced by DB included alkaline hydrolyzable conjugates (AHC), diacetyldimethoxybenzidine, monoacetyldimethoxybenzidine, and free dimethoxybenzidine. Ninety six percent of the C-14 activity, however, was non identifiable water soluble metabolites. Metabolism of DR produced AHC, monoacetyldimethylbenzidine and diacetyldimethylbenzidine. The largest amounts of C-14 activity derived from both dyes were accumulated and retained in the liver, kidney, and lung. Similar distribution patterns were seen with the free amines. The authors note that both dyes accumulate in the liver, a known target organ for rats dosed with benzidine and the site of tumors in rats dosed with benzidine based azo dyes. [Description provided by NIOSH]
• Subjects:
  Chromatography Energy Metabolism Environmental Health Laboratories Mass Spectrometry Neoplasms Occupational Health Safety
• Keywords:
  Laboratory-animals; Analytical-instruments; Monitors; Chromatographic-analysis; Cancer; Environmental-factors; Laboratory-equipment; Mass-spectrometry; Analytical-methods; Metabolites
• Document Type:
  Text
• Genre:
  Proceedings
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  426-436
• NIOSHTIC Number:
  nn:00157975
• Citation:
  NIOSH 1984:426-436
• CAS Registry Number:
  Dimethoxybenzidine (CAS RN 1331-53-9) ; Direct Blue 15 (CAS RN 2429-74-5) ; o-Tolidine (CAS RN 119-93-7) ; Rirect Red 2 (CAS RN 992-59-6)
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  False
• Source Full Name:
  Proceedings of the Third NCI/EPA/NIOSH Collaborative Workshop: Progress on Joint Environmental and Occupational Cancer Studies
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/242162/cdc_242162_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]