Targeted DNA Demethylation and Endogenous Gene Activation Using Programmable TALE-TET1 Fusions

Maeder, Morgan L; Angstman, James F; Richardson, Marcy E; Linder, Samantha J; Cascio, Vincent M; Tsai, Shengdar Q; Ho, Quan H; Sander, Jeffry D; Reyon, Deepak; Bernstein, Bradley E; Costello, Joseph F; Wilkinson, Miles F; Joung, J Keith

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Targeted DNA Demethylation and Endogenous Gene Activation Using Programmable TALE-TET1 Fusions

Published Date:

Oct 09 2013

Source:

Nat Biotechnol. 31(12):1137-1142.

[PDF-1.10 MB]

Details:

Alternative Title:

Nat Biotechnol

Personal Author:

Maeder, Morgan L ; Angstman, James F ; Richardson, Marcy E ; Linder, Samantha J ; Cascio, Vincent M ; ... More ▼

Description:

Genome-wide studies have defined cell type-specific patterns of DNA methylation that are important for regulating gene expression in both normal development and disease. However, determining the functional significance of specific methylation events remains challenging, owing to the lack of methods for removing such modifications in a targeted manner. Here we describe an approach for efficient targeted demethylation of specific CpGs in human cells using fusions of engineered transcription activator-like effector (TALE) repeat arrays and the TET1 hydroxylase catalytic domain. Using these TALE-TET1 fusions, we demonstrate that modification of critical methylated promoter CpG positions can lead to substantial increases in the expression of endogenous human genes. Our results delineate a strategy for understanding the functional significance of specific CpG methylation marks in the context of endogenous gene loci and validate programmable DNA demethylation reagents with potential utility for research and therapeutic applications.

Subject:

[+]

Pubmed ID:

24108092

Pubmed Central ID:

PMC3858462

Document Type:

Journal Article

Funding:

DP1 GM105378/DP/NCCDPHP CDC HHS/United States ; DP1 GM105378/GM/NIGMS NIH HHS/United States ; F32 GM105189/GM/NIGMS NIH HHS/United States ; F32 GM105189/GM/NIGMS NIH HHS/United States ; P30 NS45776/NS/NINDS NIH HHS/United States ; ... More ▼

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CDC Public Access

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