NanoSpotTM Collector for Aerosol Sample Collection for Direct Microscopy and Spectroscopy Analysis (Dataset)

Details

• Corporate Authors:
  National Institute for Occupational Safety and Health
• Description:
  We describe design and characterization of an aerosol NanoSpotTM collector, designed for collection of airborne particles on a microscopy substrate for direct electron microscopy, optical microscopy, and laser spectroscopy analysis. The collector implements a water-based, laminar-flow, condensation growth technique, followed by impaction onto an optical/electron microscopy substrate or a transmission electron microscopy grid for direct analysis. The compact design employs three parallel growth tubes allowing a sampling flow rate of 1.2 L min-1. Each of the three growth tubes consists of three-temperature regions, for controlling the water vapor saturation profile and exit dew point. Following the droplet growth, the three streams merge into one flow and a converging nozzle enhances focusing of the grown droplets into a tight beam, prior to their final impaction on the warm surface of the collection substrate. A miniscule sample deposition area is attained for effective coupling with microscopic and spectroscopic analysis. Experiments were conducted for the acquisition of the size-dependent collection efficiency, the uniformity of the spot deposit, the surface density distribution of the collected particles, and the aerosol concentration effect on the triple-tube NanoSpotTM Collector. Particles as small as 7 nm could be activated and collected on the electron microscopy stub. A spot deposit of approximately 0.7-mm diameter is formed for particles over a broad particle diameter range. The collected particle samples were analyzed using electron microscopy and Raman spectroscopy for the acquisition of the particle spatial distribution, the spot sample uniformity, and the analyte concentration. Finally, the NanoSpotTM collector's analytical measurement sensitivity for laser Raman analysis and the counting statistics for fiber count measurement using optical microscopy were calculated and were compared with those of the conventional aerosol sampling methods. [Description provided by NIOSH]
• Subjects:
  Aerosols Environmental Monitoring Microbiology Occupational Health Safety
• Keywords:
  Aerosol Particles Aerosol Sampling Air Sampling Techniques Electron Microscopy Equipment Design Microscopic Analysis Nanotechnology Sampling Equipment
• DOI:
  https://doi.org/10.26616/NIOSHRD-1058-2023-0
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Dataset
• Genre:
  Research Dataset
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20067081
• Citation:
  Cincinnati, OH: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1058-2023-0, 2023 Mar; :dataset
• Contact Point Address:
  Health Effects Laboratory Division (HELD), National Institute for Occupational Safety and Health (NIOSH), Cincinnati, OH. Phone: 513-533-6800
• Federal Fiscal Year:
  2023
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  NanoSpotTM collector for aerosol sample collection for direct microscopy and spectroscopy analysis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:df44771d05b8fe9853b8cf6cbb058aab363263fac14d929e6c71232e79f7012fede13085a13620ddc32de897ac145f3add465103c8937747c4d86f64c0d84558
• Download URL:
  https://stacks.cdc.gov/view/cdc/231279/cdc_231279_DS1.pdf
• File Type:
  [PDF - 188.37 KB ]