Development of a Size-Selective Sampler Combined with an Adenosine Triphosphate Bioluminescence Assay for the Rapid Measurement of Bioaerosols

Details

• Personal Author:
  Byeon JH ; Liao L ; Park JH
• Description:
  In this study, a size-selective bioaerosol sampler was built and combined with adenosine triphosphate (ATP) bioluminescence assay for measuring the bioaerosol concentration more rapidly and easily. The ATP bioaerosol sampler consisted of a respirable cyclone, an impactor to collect bioaerosols onto the head of a swab used for ATP bioluminescence assay, a swab holder, and a sampling pump. The collection efficiency of the impactor was tested using aerosolized sodium chloride particles and then the particle diameter corresponding to the collection efficiency of 50% (cut-off diameter) was evaluated. The experimental cut-off diameter was 0.44 µm. The correlations between ATP bioluminescence (relative light unit; RLU) from commercially available swabs (UltraSnap and SuperSnap, Hygiena, LLC, U.S.A.) and colony forming unit (CFU) were examined using Escherichia coli (E. coli) suspension and then the conversion equations from RLU to CFU were obtained. From the correlation results, the R2 values of UltraSnap and SuperSnap were 0.53 and 0.81, respectively. The conversion equations were the linear function and the slopes of UltraSnap and SuperSnap were 633.6 and 277.78, respectively. In the lab and field tests, the ATP bioaerosol sampler and a conventional Andersen impactor were tested and the results were compared. In the lab tests, concentrations of aerosolized E. coli collected using the sampler were highly correlated to those from the Anderson impactor (R2 = 0.85). In the field tests, the concentrations measured using the ATP bioaerosol sampler were higher than those from the Andersen impactor due to the limitations of the colony counting method. These findings confirm the feasibility of developing a sampler for rapid measurement of bioaerosol concentrations, offering a compact device for measuring exposure to bioaerosols, and an easy-to-use methodological concept for efficient air quality management. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollution Bacteria Dust Occupational Health Safety
• Keywords:
  Aerosol Particles Air Quality Measurement Area Sampling Author Keywords: Bacteria Bacterial Dusts Bioaerosols Exposure Assessment Personal Sampling Respirable Dust Sampling Equipment
• ISSN:
  0013-9351
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  194
• NIOSHTIC Number:
  nn:20068168
• Citation:
  Environ Res 2021 Mar; 194:110615
• Contact Point Address:
  Jae Hong Park, School of Health Sciences, Purdue University, West Lafayette, IN, 47906, USA
• Email:
  park895@purdue.edu
• Federal Fiscal Year:
  2021
• Performing Organization:
  Purdue University, West Lafayette, Indiana
• Peer Reviewed:
  True
• Start Date:
  20010701
• Source Full Name:
  Environmental Research
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:04ad0ca783e0f659f43af9ec42f3d85fd6ed910263f704fd52e350beabb2696b6d58f320a7225dc2516dff28e93df978ed5d77674a46c43c40e18730b18830e2
• Download URL:
  https://stacks.cdc.gov/view/cdc/230380/cdc_230380_DS1.pdf
• File Type:
  [PDF - 4.20 MB ]