Biologically Synthesized Zinc and Copper Oxide Nanoparticles Using Cannabis sativa L. Enhance Soybean (Glycine max) Defense Against Fusarium virguliforme

Details

• Personal Author:
  Abdelraheem W ; Barroso KA ; Bharadwaj A ; da Silva W ; Dimkpa C ; Elmer WH ; Karmous I ; Milagres J ; Vaidya S ; White, Jack C. ; Zuverza-Mena N
• Description:
  In this study, zinc and copper oxide nanoparticles (NPs) were synthesized using hemp (Cannabis sativa L.) leaves (ZnONP-HL and CuONP-HL), and their antifungal potential was assessed against Fusarium virguliforme in soybean (Glycine max L.). Hemp was selected because it is known to contain large quantities of secondary metabolites that can potentially enhance the reactivity of NPs through surface property modification. Synthesizing NPs with biologically derived materials allows to avoid the use of harsh and expensive synthetic reducing and capping agents. The ZnONP-HL and CuONP-HL showed average grain/crystallite size of 13.51 nm and 7.36 nm, respectively. The biologically synthesized NPs compared well with their chemically synthesized counterparts (ZnONP chem, and CuONP chem; 18.75 nm and 10.05 nm, respectively), confirming the stabilizing role of hemp-derived biomolecules. Analysis of the hemp leaf extract and functional groups that were associated with ZnONP-HL and CuONP-HL confirmed the presence of terpenes, flavonoids, and phenolic compounds. Biosynthesized NPs were applied on soybeans as bio-nano-fungicides against F. virguliforme via foliar treatments. ZnONP-HL and CuONP-HL at 200 µg/mL significantly (p < 0.05) increased (approx. 50%) soybean growth, compared to diseased controls. The NPs improved the nutrient (e.g., K, Ca, P) content and enhanced photosynthetic indicators of the plants by 100-200%. A 300% increase in the expression of soybean pathogenesis related GmPR genes encoding antifungal and defense proteins confirmed that the biosynthesized NPs enhanced disease resistance against the fungal phytopathogen. The findings from this study provide novel evidence of systemic suppression of fungal disease by nanobiopesticides, via promoting plant defense mechanisms. [Description provided by NIOSH]
• Subjects:
  Cannabis Energy Metabolism Fungi Fungicide, Industrial Insecticides Occupational Health Safety
• Keywords:
  Antifungals Author Keywords: Antifungal Biopesticide Cannabis Sativa L. Copper Oxide Copper Oxide Nanoparticles Fungicides Fusarium Virguliforme Metabolites Nanoparticles Pesticides Zinc Oxide Zinc Oxide Nanoparticles

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• ISSN:
  0048-3575
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; Ohio ; OSHA Region 1 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  194
• NIOSHTIC Number:
  nn:20067904
• Citation:
  Pestic Biochem Physiol 2023 Aug; 194:105486
• Contact Point Address:
  Ines Karmous, The Higher Institute of Applied Biology of Medenine (ISBAM), University of Gabes, Tunisia
• Email:
  ineskarmouss@gmail.com
• CAS Registry Number:
  Copper oxide (Cu2O) (CAS RN 1317-39-1) ; Zinc oxide (ZnO) (CAS RN 1314-13-2)
• Federal Fiscal Year:
  2023
• Peer Reviewed:
  True
• Source Full Name:
  Pesticide Biochemistry and Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7466f436f37c14586062c8f084015e33523acc2c0e268a666dd4a1f339d7ece7d9b7b5dec97666167a419b4b4da95bd7b506c81325b33e1cf98060fe7be2aa42
• Download URL:
  https://stacks.cdc.gov/view/cdc/230139/cdc_230139_DS1.pdf
• File Type:
  [PDF - 21.68 MB ]