Abnormalities in the Male Reproductive System After Exposure to Diesel and Biodiesel Blend

Details

• Personal Author:
  Bugarski, Aleksandar D. ; Farcas MT ; Gutkin DW ; Kagan VE ; Kisin ER ; Shurin MR ; Shvedova AA ; Yanamala N
• Description:
  Altering the fuel source from petroleum-based ultra-low sulfur diesel to biodiesel and its blends is considered by many to be a sustainable choice for controlling exposures to particulate material. As the exhaust of biodiesel/diesel blends is composed of a combination of combustion products of polycyclic aromatic hydrocarbons and fatty acid methyl esters, we hypothesize that 50% biodiesel/diesel blend (BD50) exposure could induce harmful outcomes because of its ability to trigger oxidative damage. Here, adverse effects were compared in murine male reproductive organs after pharyngeal aspiration with particles generated by engine fueled with BD50 or neat petroleum diesel (D100). When compared with D100, exposure to BD50 significantly altered sperm integrity, including concentration, motility, and morphological abnormalities, as well as increasing testosterone levels in testes during the time course postexposure. Serum level of luteinizing hormone was significantly depleted only after BD50 exposure. Moreover, we observed that exposure to BD50 significantly increased sperm DNA fragmentation and the upregulation of inflammatory cytokines in the serum and testes on Day 7 postexposure when compared with D100. Histological evaluation of testes sections from BD50 exposure indicated more noticeable interstitial edema, degenerating spermatocytes, and dystrophic seminiferous tubules with arrested spermatogenesis. Significant differences in the level of oxidative stress assessed by accumulation of lipid peroxidation products and depletion of glutathione were detected on exposure to respirable BD50 and D100. Taken together, these results indicate that exposure of mice to inhalable BD50 caused more pronounced adverse effects on male reproductive function than diesel. [Description provided by NIOSH]
• Subjects:
  DNA Endocrine System Environmental Monitoring Esters Fuel Oils Hydrocarbons Immune System Laboratories Men Occupational Health Petroleum Polycyclic Aromatic Hydrocarbons Safety Spermatogenesis Urogenital Diseases Urogenital System Vehicle Emissions

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• Keywords:
  Author Keywords: Pulmonary Exposure Biodiesel Particles Combustion-products Diesel-emissions Diesel-exhausts DNA-damage DNA Fragmentation Exposure-assessment Fatty-acid-esters Fatty-acids Fuel-production Fuels Hormone-activity Immune-reaction Laboratory-animals Laboratory-testing Lipid-peroxidation Male Reproduction Methyl-compounds Morphology Oxidative-processes Oxidative Stress Petroleum-products Polycyclic-aromatic-hydrocarbons Reproduction Reproductive-effects Reproductive-hazards Reproductive-system Reproductive-system-disorders Sperm Quality Testes

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• Series:
  Mining Publications
• ISSN:
  0893-6692
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; OMSHR - Office of Mine Safety and Health Research
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  265-276
• Volume:
  56
• Issue:
  2
• NIOSHTIC Number:
  nn:20045277
• Citation:
  Environ Mol Mutagen 2015 Mar; 56(2):265-276
• Contact Point Address:
  Anna A. Shvedova, Pathology and Physiology Research Branch, and Exposure Assessment Branch, HELD, NIOSH, 1095 Willowdale Rd, Morgantown, WV 26505, USA
• Email:
  ats1@cdc.gov
• CAS Registry Number:
  Glutathione (CAS RN 70-18-8)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Mining ; Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Environmental and Molecular Mutagenesis
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f5e18b8716ec26e34b553cd1baa2612d619624d776f9f17912756bf5da5aba6dfdbf40e5eab8e4f5b85ede4219d427bb0a99488c191b5ef6c3a7151a051bb156
• Download URL:
  https://stacks.cdc.gov/view/cdc/228069/cdc_228069_DS1.pdf
• File Type:
  [PDF - 350.24 KB ]