Effects of Occupational Nanomaterial Exposure on the Blood Brain Barrier

Details

• Personal Author:
  Carter D ; Muldoon P ; Ottens AK ; Pilaka P ; Saunders SA
• Description:
  With the increasing use of carbon nanotubes (CNT) in automobile, aerospace, sport equipment, construction, and plastic industries, concern over negative implications for worker's health is growing. Recent evidence has shown that exposure to CNT can cause inflammation and fibrosis in the lungs and induce vascular dysfunction. Inhalation of CNT may also indirectly perturb the blood brain-barrier resulting in bioactive and potentially neurotoxic molecules crossing into the brain. It has yet to be determined how active biomolecules released in response to the inhalation of CNTs affects the integrity of the blood-brain barrier and induce cognitive deficits or enhance dementia. The focus of this project is to assess if larger bio molecules such as albumin are crossing the barrier and gaining access to the brain as a response to the inhalation of multi-walled CNT. To assess effects on the blood-brain barrier, mice were exposed to different doses of MWCNT by aspiration: 0 µg (vehicle only), 10 µg, and 40 µg. Mouse brains were collected and flash frozen at -80 degrees 4 h after exposure. Brains were then sagittally cryosectioned from the midline and surveyed by immunofluorescence to assess damaged blood vessels. The hippocampus and Thalamus regions showed increased vulnerability to blood vessel damage as compared to other regions. Notably, extravascular albumin within 10 µg sections demonstrated a distinct structure that resembled glial morphology. Future directions will further investigate differential glial response that may be associated with extravascular clean-up that is unique to the 10 µg dose, which may underlie the genesis of a chronic neuroinflammatory response. [Description provided by NIOSH]
• Subjects:
  Animals Occupational Exposure Occupational Health Safety
• Keywords:
  Animal Studies Health Effects Multi-walled Carbon Nanotubes MWCNT Nanomaterials Neurological System Occupational Exposure
• Publisher:
  Virginia Commonwealth University
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  New Mexico ; OSHA Region 3 ; OSHA Region 6 ; Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1
• NIOSHTIC Number:
  nn:20063326
• Citation:
  3rd Annual American Society for Biochemistry and Molecular Biology ASBMB-VA 2016 Undergraduate Research Conference, October 14, Richmond, Virginia. Richmond, VA: Virginia Commonwealth University, 2016 Oct; :1
• CAS Registry Number:
  Carbon nanotubes (CAS RN 308068-56-6)
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of New Mexico Health Sciences Center, Albuquerque, New Mexico
• Peer Reviewed:
  False
• Start Date:
  20150930
• Source Full Name:
  3rd Annual American Society for Biochemistry and Molecular Biology ASBMB-VA 2016 Undergraduate Research Conference, October 14, Richmond, Virginia
• End Date:
  20190929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:dcf84eb94d03bdbe930b1f3d7c114e5ba5b054b145fe224d94177f5a66d327ce9504688761903ec92fc6b7c86f6c61453f98eafe3a73c47412eba80a59601203
• Download URL:
  https://stacks.cdc.gov/view/cdc/226405/cdc_226405_DS1.pdf
• File Type:
  [PDF - 220.30 KB ]