Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation

Details

• Alternative Title:
  Nucleic Acids Res
• Personal Author:
  Pavon-Eternod, Mariana ; David, Alexandre ; Dittmar, Kimberly ; Berglund, Peter ; Pan, Tao ; Bennink, Jack R. ; Yewdell, Jonathan W.
• Description:
  Transfer RNAs (tRNAs) are central to protein synthesis and impact translational speed and fidelity by their abundance. Here we examine the extent to which viruses manipulate tRNA populations to favor translation of their own genes. We study two very different viruses: influenza A virus (IAV), a medium-sized (13 kB genome) RNA virus; and vaccinia virus (VV), a large (200 kB genome) DNA virus. We show that the total cellular tRNA population remains unchanged following viral infection, whereas the polysome-associated tRNA population changes dramatically in a virus-specific manner. The changes in polysome-associated tRNA levels reflect the codon usage of viral genes, suggesting the existence of local tRNA pools optimized for viral translation.
• Subjects:
  Codon Genes, Viral HeLa Cells Humans Influenza A Virus Interferons Polyribosomes Protein Biosynthesis RNA RNA, Transfer Vaccinia Virus Virus Replication

  More +
• Source:
  Nucleic Acids Res. 2012; 41(3):1914-1921.
• Pubmed ID:
  23254333
• Pubmed Central ID:
  PMC3561966
• Document Type:
  Journal Article
• Funding:
  DP1 GM105386/DP/NCCDPHP CDC HHS/United States ; T32 GM007183/GM/NIGMS NIH HHS/United States
• Volume:
  41
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:53e085f0323e5ad54ca29d128307adef825efa48bcc1ea37d35b50a8a60d513d
• Download URL:
  https://stacks.cdc.gov/view/cdc/22637/cdc_22637_DS1.pdf
• File Type:
  [PDF - 3.43 MB ]