Details:

Alternative Title:
Nucleic Acids Res
Publisher's site:
http://dx.doi.org/10.1093/nar/gks986
Personal Author:
Pavon-Eternod, Mariana ; David, Alexandre ; Dittmar, Kimberly ; Berglund, Peter ; Pan, Tao ; ... More +
Pavon-Eternod, Mariana ; David, Alexandre ; Dittmar, Kimberly ; Berglund, Peter ; Pan, Tao ; Bennink, Jack R. ; Yewdell, Jonathan W. Less -
Description:
Transfer RNAs (tRNAs) are central to protein synthesis and impact translational speed and fidelity by their abundance. Here we examine the extent to which viruses manipulate tRNA populations to favor translation of their own genes. We study two very different viruses: influenza A virus (IAV), a medium-sized (13 kB genome) RNA virus; and vaccinia virus (VV), a large (200 kB genome) DNA virus. We show that the total cellular tRNA population remains unchanged following viral infection, whereas the polysome-associated tRNA population changes dramatically in a virus-specific manner. The changes in polysome-associated tRNA levels reflect the codon usage of viral genes, suggesting the existence of local tRNA pools optimized for viral translation.
Subject:
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Codon
Genes, Viral
HeLa Cells
Humans
Influenza A Virus
Interferons
Polyribosomes
Protein Biosynthesis
RNA
RNA, Transfer
Vaccinia Virus
Virus Replication

Source:
Nucleic Acids Res. 2012; 41(3):1914-1921.
Pubmed ID:
23254333
Pubmed Central ID:
PMC3561966
Document Type:
Journal Article
Funding:
DP1 GM105386/DP/NCCDPHP CDC HHS/United States ; T32 GM007183/GM/NIGMS NIH HHS/United States
DP1 GM105386/DP/NCCDPHP CDC HHS/United States ; T32 GM007183/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:53e085f0323e5ad54ca29d128307adef825efa48bcc1ea37d35b50a8a60d513d
File Type:

Supporting Files

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