Two-photon calcium imaging during fictive navigation in virtual environments

Ahrens, Misha B.; Huang, Kuo Hua; Narayan, Sujatha; Mensh, Brett D.; Engert, Florian

doi:10.3389/fncir.2013.00104

i

Two-photon calcium imaging during fictive navigation in virtual environments

Supporting Files

Jun 06 2013
By Ahrens, Misha B. ; Huang, Kuo Hua ; Narayan, Sujatha ; ...

Details

Alternative Title:

Front Neural Circuits
Personal Author:

Ahrens, Misha B. ; Huang, Kuo Hua ; Narayan, Sujatha ; Mensh, Brett D. ; Engert, Florian
Description:

A full understanding of nervous system function requires recording from large populations of neurons during naturalistic behaviors. Here we enable paralyzed larval zebrafish to fictively navigate two-dimensional virtual environments while we record optically from many neurons with two-photon imaging. Electrical recordings from motor nerves in the tail are decoded into intended forward swims and turns, which are used to update a virtual environment displayed underneath the fish. Several behavioral features-such as turning responses to whole-field motion and dark avoidance-are well-replicated in this virtual setting. We readily observed neuronal populations in the hindbrain with laterally selective responses that correlated with right or left optomotor behavior. We also observed neurons in the habenula, pallium, and midbrain with response properties specific to environmental features. Beyond single-cell correlations, the classification of network activity in such virtual settings promises to reveal principles of brainwide neural dynamics during behavior.
Subjects:

Animals Avoidance Learning Behavior Calcium Signaling Environment Larva Microscopy, Fluorescence, Multiphoton Motor Control Neurons Neuroscience Rhombencephalon Sensorimotor Transformations Swimming Two-photon Calcium Imaging User-Computer Interface Virtual Reality Zebrafish
Source:

Front Neural Circuits. 2013; 7.
Pubmed ID:

23761738
Pubmed Central ID:

PMC3674334
Document Type:

Journal Article
Funding:

DP1 NS082121/DP/NCCDPHP CDC HHS/United States ; R01 DA030304/DA/NIDA NIH HHS/United States ; Howard Hughes Medical Institute/United States ; Wellcome Trust/United Kingdom
Volume:

7
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:76e4bc9c0d930a7a4dfc26813e1bfab9822553ee6f32b9e7b481e193f1b7a56f
Download URL:

https://stacks.cdc.gov/view/cdc/22604/cdc_22604_DS1.pdf
File Type:

[PDF - 4.63 MB ]

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