Method Validation for Measurement of Hair Nicotine Level in Nonsmokers

Details

• Personal Author:
  Avila-Tang E ; Breysse PN ; Kim SR ; Samet JM ; Wipfli H
• Description:
  The development of strategies to address the growing worldwide burden of exposure to secondhand smoke (SHS) would be facilitated by sensitive and accurate methods for assessing SHS exposure. Hair provides a readily available matrix for assessing biomarkers of typical SHS exposure. We developed and applied an optimized analytical method using an isotope dilution gas chromatography-mass spectrometry (GC/MS) for hair nicotine measurement. The utility of this optimized method is illustrated by presenting data on SHS exposure of women and children from 31 countries. Using this isotope dilution method with spiked samples (3.3 ng/mg), we found that the greatest hair nicotine extraction efficiency was obtained with a 60 min shaking time. In the field study (n = 2400), a positive association was evident between hair nicotine concentrations from nonsmokers and higher numbers of cigarettes smoked per day in a household. [Description provided by NIOSH]
• Subjects:
  Laboratories Nicotine Occupational Health Safety Women
• Keywords:
  Author Keywords: Nicotine Children Gas Chromatography Gas Chromatography-mass Spectrometry Hair Hair Analysis Laboratory Techniques Mass Spectrometry Secondhand Smoke
• ISSN:
  0269-3879
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  273-279
• Volume:
  23
• Issue:
  3
• NIOSHTIC Number:
  nn:20062779
• Citation:
  Biomed Chromatogr 2009 Mar; 23(3):273-279
• Contact Point Address:
  Sung Roul Kim, 615 N. Wolf Street W7010H, Baltimore,MD 21205, USA
• Email:
  srkim@jhsph.edu
• CAS Registry Number:
  (-)-Nicotine (CAS RN 54-11-5)
• Federal Fiscal Year:
  2009
• Performing Organization:
  Johns Hopkins University
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Biomedical Chromatography
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4cf7c9e8fc02c6ec485b8b2db2b14e268f85a61a103c9a7b8f12a4dd5530a6c79c1255b961a1c2826ed5696036574ea8ba48d6bad0785305654228d33c53e7b9
• Download URL:
  https://stacks.cdc.gov/view/cdc/225969/cdc_225969_DS1.pdf
• File Type:
  [PDF - 431.62 KB ]