Organic Dust Exposure Induces Stress Response and Mitochondrial Dysfunction in Monocytic Cells

Details

• Personal Author:
  Bhat SM ; Charavaryamath C ; Kanthasamy AG ; Karriker LA ; Massey N ; Shrestha D
• Description:
  Exposure to airborne organic dust (OD), rich in microbial pathogen-associated molecular patterns (PAMPs), is shown to induce lung inflammation. A common manifestation in lung inflammation is altered mitochondrial structure and bioenergetics that regulate mitochondrial ROS (mROS) and feed a vicious cycle of mitochondrial dysfunction. The role of mitochondrial dysfunction in other airway diseases is well known. However, whether OD exposure induces mitochondrial dysfunction remains elusive. Therefore, we tested a hypothesis that organic dust extract (ODE) exposure induces mitochondrial stress using a human monocytic cell line (THP1). We examined whether co-exposure to ethyl pyruvate (EP) or mitoapocynin (MA) could rescue ODE exposure induced mitochondrial changes. Transmission electron micrographs showed significant differences in cellular and organelle morphology upon ODE exposure. ODE exposure with and without EP co-treatment increased the mtDNA leakage into the cytosol. Next, ODE exposure increased PINK1, Parkin, cytoplasmic cytochrome c levels, and reduced mitochondrial mass and cell viability, indicating mitophagy. MA treatment was partially protective by decreasing Parkin expression, mtDNA and cytochrome c release and increasing cell viability. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Dust Lung Occupational Health Respiratory System Safety
• Keywords:
  Author Keywords: Ethyl Pyruvate Cell Function Cell Morphology Cellular Effects Cytotoxic Effects DNA Damage Electron Microscopy Exposure Assessment Lung Irritants Mitoapocynin Mitochondrial DNA Mitochondrial Dysfunction Organic Dust Organic Dusts

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• ISSN:
  0948-6143
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Iowa ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  155
• Issue:
  6
• NIOSHTIC Number:
  nn:20062303
• Citation:
  Histochem Cell Biol 2021 Jun; 155(6):699-718
• Contact Point Address:
  Chandrashekhar Charavaryamath, Department of Biomedical Sciences, Iowa State University, 2008 Vet Med Building, Ames, IA 50011, USA
• Email:
  chandru@iastate.edu
• CAS Registry Number:
  Ethyl pyruvate (CAS RN 617-35-6)
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Iowa, Iowa City
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Histochemistry and Cell Biology
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d28534a476f23d958ab5d2f16531b3c18f2e467bbb4e5fee87e80eb7c945e407963ebae763641a5dd9f8296d906f7c9214b3fd374108eda8ecd4eae10987eb85
• Download URL:
  https://stacks.cdc.gov/view/cdc/225580/cdc_225580_DS1.pdf
• File Type:
  [PDF - 3.49 MB ]