Welding Fume Inhalation Exposure and High-Fat Diet Change Lipid Homeostasis in Rat Liver

Details

• Personal Author:
  Antonini JM ; Boyce G ; Erdely A ; Kodali V ; McKinney W ; Meighan TG ; Powell MJ ; Roach KA ; Roberts, Jennifer R. ; Shoeb M ; Stone S ; Zeidler-Erdely PC
• Description:
  It is estimated that greater than one million workers are exposed to welding fume (WF) by inhalation daily. The potentially toxic metals found in WF are known to cause multiple adverse pulmonary and systemic effects, including cardiovascular disease, and these metals have also been shown to translocate to the liver. This occupational exposure combined with a high fat (HF) Western diet, which has been shown to cause hyperlipidemia and non-alcoholic fatty liver disease (NAFLD), has the potential to cause significant mixed exposure metabolic changes in the liver. Matrix assisted laser desorption ionization (MALDI) mass spectrometry allows for direct analysis of tissues for the identification and relative quantification of multiple biomolecules, including lipids. The goal of this study was to use matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) to analyze the spatial distribution and abundance changes of lipid species in Sprague Dawley rat liver maintained on a HF diet combined with WF inhalation. Male Sprague Dawley rats from each diet were exposed by inhalation to stainless steel WF at a target concentration of 20 mg/m3 for 3 hours per day for 5 weeks or filtered air as the control. The results of the MALDI-IMS analysis revealed unique hepatic lipid profiles for each treatment group at 12 weeks post-exposure. Pulmonary exposure to WF alone increased the levels of ceramide-1-phosphate and lyso-phosphatidylinositol (18:0) which are both markers of inflammation. The HF diet group had significantly increased abundance of triglycerides and phosphatidylinositol lipids, as well as decreased lysophosphatidic lipids and cardiolipin. The increased hepatic triglycerides were found in conjunction with significantly increased serum triglycerides and oil-red-O staining showed increased lipid deposition in the HF diet animals. Ceramide-1-phosphate was found at higher abundance in the regular (REG) diet WF-exposed group which has been shown to regulate the eicosanoid pathway involved in pro-inflammatory response. The results of this study showed that the combined effects of WF inhalation and a HF diet significantly altered the hepatic lipidome. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Lipids Occupational Health Respiratory System Safety Toxicology Welding
• Keywords:
  Animal Studies Dietary Effects Inhalation Studies Liver Damage Liver Tissue Mass Spectrometry Physiological Response Welding Fumes
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  180
• NIOSHTIC Number:
  nn:20062262
• Citation:
  Toxicologist 2021 Mar; 180(S1):109
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 60th Annual Meeting and ToxExpo, March 12-26, 2021, Virtual Event
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9db3d0756883aa6dc602a43ecc353e06d5b7f35fd2373e16349de45a75d4ffec0947f60ad73df74f68e6a84b4428b451b939cbfe29ba42749f11de6d9ad08804
• Download URL:
  https://stacks.cdc.gov/view/cdc/225544/cdc_225544_DS1.pdf
• File Type:
  [PDF - 141.55 KB ]