Invited Review: Human Air-Liquid-Interface Organotypic Airway Tissue Models Derived from Primary Tracheobronchial Epithelial Cells – Overview and Perspectives

Details

• Personal Author:
  Cao X ; Coyle JP ; Gwinn WM ; Hayden P ; Heflich RH ; Ren B ; Rojanasakul L ; Wang Y ; Xiong R
• Description:
  The lung is an organ that is directly exposed to the external environment. Given the large surface area and extensive ventilation of the lung, it is prone to exposure to airborne substances, such as pathogens, allergens, chemicals, and particulate matter. Highly elaborate and effective mechanisms have evolved to protect and maintain homeostasis in the lung. Despite these sophisticated defense mechanisms, the respiratory system remains highly susceptible to environmental challenges. Because of the impact of respiratory exposure on human health and disease, there has been considerable interest in developing reliable and predictive in vitro model systems for respiratory toxicology and basic research. Human air-liquid-interface (ALI) organotypic airway tissue models derived from primary tracheobronchial epithelial cells have in vivo-like structure and functions when they are fully differentiated. The presence of the air-facing surface allows conducting in vitro exposures that mimic human respiratory exposures. Exposures can be conducted using particulates, aerosols, gases, vapors generated from volatile and semi-volatile substances, and respiratory pathogens. Toxicity data have been generated using nanomaterials, cigarette smoke, e-cigarette vapors, environmental airborne chemicals, drugs given by inhalation, and respiratory viruses and bacteria. Although toxicity evaluations using human airway ALI models require further standardization and validation, this approach shows promise in supplementing or replacing in vivo animal models for conducting research on respiratory toxicants and pathogens. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Animals Dust Environmental Exposure Hypersensitivity Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Solvents Toxicology Ventilation Viral Diseases

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• Keywords:
  Airborne Dusts Airborne Particles Airborne Particulates Allergens Author Keywords: Air-liquid-interface (ALI) Airway Cultures Bacteria Cell Biology Cellular Function Chemicals Cigarette Smoke Diseases Drugs Environmental Exposure Exposure Levels Exposure System Gases Health Effects Inhalation Toxicology In Vitro Studies In Vitro Study In Vivo Studies In Vivo Study Laboratory Animals Lung Cells Models Nanomaterials Nanotechnology Particulates Pathogen-host Interaction Pathogens Pulmonary Drug Testing Pulmonary System Disorders Respiratory System Disorders Risk Factors Tissue Culture Vapors Viral Diseases Viral Infections Volatiles

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• ISSN:
  1071-2690
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Arkansas ; Massachusetts ; North Carolina ; OSHA Region 1 ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  104-132
• Volume:
  57
• Issue:
  2
• NIOSHTIC Number:
  nn:20061501
• Citation:
  In Vitro Cell Dev Biol Anim 2021 Feb; 57(2):104-132
• Contact Point Address:
  Xuefei Cao, Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Rd., Jefferson, AR, USA
• Email:
  Xuefei.cao@fda.hhs.gov
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  In Vitro Cellular & Developmental Biology. Animal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fb6ac9297ead95e3cf9d3d78f6363902b175dbdbc912e389bd45e9d9e8b4d0148123cf16383625ab2eb66450924fc8792a0d661c168ca8870eb9ffb123b92e3d
• Download URL:
  https://stacks.cdc.gov/view/cdc/225016/cdc_225016_DS1.pdf
• File Type:
  [PDF - 1.36 MB ]