Analysis of Mutagenic Activity in Cigarette Smokers’ Urine by High Performance Liquid Chromatography

Details

• Personal Author:
  Eisenstadt E ; Langley D ; Putzrath RM
• Description:
  Mutagenic activity in smokers' urine which had been concentrated by XAD-2 resin can be separated from approximately 90% of the non-mutagenic material by CH2Cl2 extraction. This extract appears to be stable for 3 months at -20 degrees C. High performance liquid chromatography analysis of the CH2Cl2 extract showed multiple mutagenic non-polar fractions which were better activated by rat liver homogenates prepared from 3-methylcholanthrene treated rats than from rats treated with phenobarbital. Mutagenic activity in smokers' urine was extractable by acid, but not by base. The mutagens in smokers' urine appear to be a complex mixture of relatively non-polar chemicals. [Description provided by NIOSH]
• Subjects:
  Animals Mutagens Occupational Health Safety Smokers Tobacco Products Urine
• Keywords:
  Animal Studies Cigarette Smoking Liquid Chromatography Mutagenesis
• ISSN:
  0027-5107
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  97-108
• Volume:
  85
• Issue:
  3
• NIOSHTIC Number:
  nn:20061154
• Citation:
  Mutat Res 1981 Jun; 85(3):97-108
• Contact Point Address:
  Eric Eisenstadt, Department of Microbiology, Harvard School of Public Health, 665 Huntington Avenue Boston, MA 02115, U.S.A.
• CAS Registry Number:
  Dichloromethane (CAS RN 75-09-2)
• Federal Fiscal Year:
  1981
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:07ab896afb6b9f6a9f074c7a3b20a43055a2fe64f2f46834be8bee07abac89578cf7ed4e4290ee48d6e5b1d53e890342ce61f9e8893f233937d4f7b1fb17042d
• Download URL:
  https://stacks.cdc.gov/view/cdc/224792/cdc_224792_DS1.pdf
• File Type:
  [PDF - 593.44 KB ]