Reversible Microsomal Binding of Hepatic Aldolase

Details

• Personal Author:
  Bernstein IA ; Weiss TL ; Zieske JD
• Description:
  Fructose-1,6-bisphosphate aldolase (d-fructose-1,6-bisphosphate d-glyceraldehyde-3-phosphate lyase, EC 4.1.2.13) partitions between the microsomes and the cytosol when a rat liver homogenate is fractionated by differential centrifugation. Gel electrophoresis and immunodiffusion indicate that the one isozyme present in the liver of the young adult rat is found in both fractions. The association of the aldolase with membranes is differentially sensitive to a variety of metabolites and inorganic salts. In the absence of cellular salts, 1 mM fructose 1,6-bisphosphate or glucose 1,6-bisphosphate elutes 50% of the enzyme from the microsomes. About 9 mM Pi or citrate is necessary to produce the same effect. With other metabolites or inorganic salts higher concentrations are required. The fraction of total enzyme which partitions with the microsomes when a homogenate is submitted to high speed centrifugation, correlates inversely with the level of fructose 1,6-bisphosphate in the supernatant solution and this concentration is higher when the tissue concentration in the homogenate is greater. The Km for fructose 1,6-bisphosphate of 3 · 10-4 for aldolase bound to microsomes is decreased to 6 · 10-6 M when the enyme is dissociated from the membranes with salt. These observations appear relevant to the ongoing discussion regarding the physiological relevance of the subcellular localization of glycolytic enzymes. [Description provided by NIOSH]
• Subjects:
  Animals Enzymes Occupational Health Safety
• Keywords:
  Animal Studies Author Keywords: Microsomal Binding Enzymatic Effects Enzyme Activity Fructose-1,6-bisphosphate Aldolase Hepatic Microsomal Enzymes Liver Enzymes Rat Liver
• ISSN:
  0006-3002
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  221-229
• Volume:
  661
• Issue:
  2
• NIOSHTIC Number:
  nn:20061143
• Citation:
  Biochim Biophys Acta 1981 Oct; 661(2):221-229
• Federal Fiscal Year:
  1982
• Performing Organization:
  University of Michigan, Ann Arbor, Michigan
• Peer Reviewed:
  True
• Start Date:
  19710701
• Source Full Name:
  Biochimica et Biophysica Acta
• End Date:
  19810630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8dbb6bf3a6a371a22913acdae61328dbca27b4e63dac7a2a16cfcbcd979d3016dce6bd0af84981e08b0f93b8c3b47ed98ec7cb87c316b4587ac18e6a5d2dc148
• Download URL:
  https://stacks.cdc.gov/view/cdc/224785/cdc_224785_DS1.pdf
• File Type:
  [PDF - 854.15 KB ]