Destruction of Microsomal Cytochrome P-450 by Reactive Oxygen Species Generated During Photosensitization of Hematoporphyrin Derivative

Details

• Personal Author:
  Bickers DR ; Dixit R ; Mukhtar H
• Description:
  Rat liver microsomal cytochrome P-450 undergoes rapid destruction in the presence of hematoporphyrin derivative (HpD) and solar radiation (approx. 400 nm). Destruction of cytochrome P-450 is associated with the formation of cytochrome P-420 and significant loss of microsomal haem. Quenchers of singlet oxygen including 2,5-dimethylfuran, histidine, ß-carotene, and ascorbic acid and inhibitors of the hydroxyl radical such as benzoate, mannitol, and ethanol prevent deterioration of the microsomal haem-protein, whereas superoxide dismutase and catalase are ineffective in this regard. These results indicate that generation of singlet oxygen during hematoporphyrin photosensitization is associated with destruction of microsomal cytochrome P-450 and haem. [Description provided by NIOSH]
• Subjects:
  Animals Enzymes Occupational Health Oxidants Safety
• Keywords:
  Animal Studies Enzymatic Effects Enzyme Activity Oxidative Processes Photosensitivity Reactive Oxygen Species ROS
• ISSN:
  0031-8655
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  173-176
• Volume:
  37
• Issue:
  2
• NIOSHTIC Number:
  nn:20060836
• Citation:
  Photochem Photobiol 1983 Feb; 37(2):173-176
• Contact Point Address:
  David R. Bickers, Department of Dermatology, Case Western Reservce University and Veterans Administration Medical Center, 10701 East Boulevard, Cleveland, OH, 44106
• Federal Fiscal Year:
  1983
• Performing Organization:
  Case Western Reserve University, Cleveland, Ohio
• Peer Reviewed:
  True
• Start Date:
  19800929
• Source Full Name:
  Photochemistry and Photobiology
• End Date:
  19830831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2c429d30ff0bdc3f797648e4081d677ce38193ee6ac312c5dbaf776261c36bf1318ba35f2fee15b6c304fbdb563661fdd55287dc25b710ec5ff7528953a6acf1
• Download URL:
  https://stacks.cdc.gov/view/cdc/224633/cdc_224633_DS1.pdf
• File Type:
  [PDF - 347.74 KB ]