Elastolysis of Insoluble Elastin

Details

• Personal Author:
  Gerblich A ; Peterson L ; Schuyler M ; Urda G
• Description:
  We developed an assay for measurement of elastolytic activity using insoluble 3H-labelled particulate elastin adherent to plastic that is capable of detecting 150 picograms of pancreatic elastase. This equals or exceeds the sensitivity of the most sensitive previously reported systems, without requiring sodium dodecyl sulfate treatment of the elastin. Elastin digestion is dependent upon substrate and elastase concentration, but is not linearly related to time. This is partially attributable to elastase denaturation or autolysis under the assay conditions. The assay could easily detect elastase secreted by either peritoneal or alveolar macrophages. Compared to previously described assays using substrates that closely resemble the physiologic substrate, this represents a considerable increase of sensitivity of detection of elastolytic activity of enzymes. [Description provided by NIOSH]
• Subjects:
  Biological Assay Blood Enzymes Lung Macrophages Occupational Health Respiratory System Safety
• Keywords:
  Alveolar Cells Bioassays Enzyme Activity Lung Tissue
• ISSN:
  0300-8207
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  87-95
• Volume:
  12
• Issue:
  2
• NIOSHTIC Number:
  nn:20060680
• Citation:
  Connect Tissue Res 1984 Apr; 12(2):87-95
• Contact Point Address:
  Mark Schuyler, Cleveland VA Medical Center, 10701 East Blvd. Cleveland, Ohio 44106
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  True
• Source Full Name:
  Connective Tissue Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ae0060576640bdd7a3a0b00dbaf504e7cfb2cd5f987508f4d2e9c4d6e806a421b222bb0bf0342e3ea49b7b11fe35c3214b22332293d4c0bea6d2a960229c26d9
• Download URL:
  https://stacks.cdc.gov/view/cdc/224520/cdc_224520_DS1.pdf
• File Type:
  [PDF - 622.40 KB ]