Increased Epidermal Transglutaminase Activity Following 2,3, 7,8-Tetrachlorodibenzo-P-Dioxin: In Vivo and In Vitro Studies with Mouse Skin

Details

• Personal Author:
  Ertl DC ; Lynberg CA ; Puhvel SM
• Description:
  In previous studies it has been shown that topical treatment of hairless mice with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin) induces hyperproliferation and hyperkeratinization in the epidermis of hairless mice. The present investigation demonstrated that such TCDD-induced morphological changes in skin in vivo are accompanied by increased levels in activity of epidermal transglutaminase (ETG), the enzyme associated with terminal epidermal differentiation. Exposure of mouse epidermal cells in tissue culture to 10-9 m TCDD also resulted in a significant increase in ETG activity, despite the fact that morphologically these cultures (grown at 0.07 mm ionic calcium concentrations) exhibited no signs of terminal differentiation. Thus one mechanism of action of TCDD in inducing cutaneous changes appears to relate to the stimulation of increased ETG levels. [Description provided by NIOSH]
• Subjects:
  Animals Enzymes Occupational Health Safety Skin
• Keywords:
  2,3,7,8-Tetrachloro-dibenzo-p-dioxin Animal Studies Dermal Exposures Dioxin Enzymatic Effects Skin Exposure TCDD
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  42-47
• Volume:
  73
• Issue:
  1
• NIOSHTIC Number:
  nn:20060665
• Citation:
  Toxicol Appl Pharmacol 1984 Mar; 73(1):42-47
• Contact Point Address:
  S.M. Puhvel, Division of Dermatology, Department of Medicine, School of Medicine, Center for Health Sciences, University of California, Los Angeles, Los Angeles, California 90024 USA
• CAS Registry Number:
  2,3,7,8-Tetrachlorodibenzo-p-dioxin (CAS RN 1746-01-6)
• Federal Fiscal Year:
  1984
• Performing Organization:
  University of California Los Angeles, Los Angeles, California
• Peer Reviewed:
  True
• Start Date:
  19800929
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19870630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3f1851941ae97095389cee0c6acbc38a71fe1b71703dfde5fddf70fb8e868dd60f759691cd119f6c340da7121f56be5db77fd2eb10b019121070b8f59b214477
• Download URL:
  https://stacks.cdc.gov/view/cdc/224509/cdc_224509_DS1.pdf
• File Type:
  [PDF - 442.74 KB ]