In Vitro Effect of N-Hexane and Its Metabolites on Selected Enzymes in Glycolysis, Pentose Phosphate Pathway and Citric Acid Cycle

Details

• Personal Author:
  Sabri MI
• Description:
  The effect of n-hexane, 2-hexanol, 5-hydroxy-2-hexanone, 2,5-hexanediol, methyln-butyl ketone (MnBK) and 2,5-hexanedione (2,5-HD) has been studied in vitro on crystalline glyceraldehyde-3-phosphate dehydrogenase (GAPDH),dl-glyceraldehyde-3-phosphate: NAD oxidoreductase (phosphorylating) EC. 1.2.1.12 and phosphofrucktokinase (PFK) ATP:d-fructose-6-phosphate-1-phosphotransferase; EC. 2.7.1.11 and lactic dehydrogenase (LDH)l-lactate: NAD+ oxidoreductase, EC. 1.1.1.27.MnBK and 2,5-HD both inhibited GAPDH and PFK activities selectively. n-Hexane and 2-hexanol had no effect on GAPDH and PFK activities; 5-hydroxy-2-hexanone and 2,5-hexanediol exhibited a slight inhibitory effect on these enzymes. Neither metabolites of n-hexane have any effect on LDH activity. 2,5-Hexanedione did not inhibit transketolase (d-sedoheptulose-7-phosphate:d-glyceraldehyde-3-phosphate glycolaldehyde transferase, EC. 2.2.1.1.) and succinate dehydrogenase (succinate: 2,6-dichlorophenol-indophenol oxidoreductase, EC. 1.3.99.1) activities. The levels of ATP were reduced in 2,5-HD-treated cat sciatic nerves and returned to normal levels by exposing the nerve to sodium pyruvate. [Description provided by NIOSH]
• Subjects:
  Animals Nervous System Occupational Health Safety Toxicology
• Keywords:
  Animal Studies Author Keywords: 2,5-hexanedione Distal Axonopathy Glyceraldehyde-3-phosphate Dehydrogenase Glycolysis Nerve Damage Nervous System Neuropathology Neuropathy Neurotoxic Effects Peripheral Neuropathy Sciatic Nerve Toxic Effects Toxic Neuropathy

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• ISSN:
  0006-8993
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  145-150
• Volume:
  297
• Issue:
  1
• NIOSHTIC Number:
  nn:20060643
• Citation:
  Brain Res 1984 Apr; 297(1):145-150
• Contact Point Address:
  M.I. Sabri, Institute of Neurotoxicology, Rose F. Kennedy Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, U.S.A.
• CAS Registry Number:
  (±)-2-Hexanol (CAS RN 626-93-7) ; 2,5-Hexanediol (CAS RN 2935-44-6) ; Hexane (CAS RN 110-54-3)
• Federal Fiscal Year:
  1984
• Performing Organization:
  Yeshiva University, New York, New York
• Peer Reviewed:
  True
• Start Date:
  19751101
• Source Full Name:
  Brain Research
• End Date:
  19850331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2a1b1144a5170d246f5cbfdf47788ecbf6a6f49e1aa37b3a517b258d3ac328f1ae2a7490e0a66c392e30de4526269b71eb7fdacb1b6003900d5dfb158b8d9eee
• Download URL:
  https://stacks.cdc.gov/view/cdc/224493/cdc_224493_DS1.pdf
• File Type:
  [PDF - 520.57 KB ]