Application of an In Vitro Keratinization Assay to Extracts of Soot from a Fire in a Polychlorinated Biphenyl-Containing Transformer

Details

• Personal Author:
  Crane D ; Frenkel GD ; Gierthy JF
• Description:
  A fire in the State Office Building in Binghamton, New York, involving a polychlorinated biphenyl-containing electrical transformer, resulted in contamination of the structure with soot containing 2,3,7,8-tetrachlorodibenzo-p-dioxin and 2,3,7,8-tetrachlorodibenzofuran. Benzene extracts of soot collected from various areas of the building were tested for in vitro keratinization-inducing activity by the method of J. C. Knutson and A. Poland (Cell 22, 27-36, 1980). The results, in terms of relative keratinization-inducing activity, are compared to a high-resolution gas chromatographic/mass spectrometric analysis for total polychlorinated dibenzofurans in the same samples. This comparison showed a good correlation and suggests that the in vitro keratinization model has potential for use as a semiquantitative assay for dioxinlike activity. [Description provided by NIOSH]
• Subjects:
  Biological Assay Dioxins Fires Occupational Health Polychlorinated Biphenyls Safety
• Keywords:
  2,3,7,8-Tetrachloro-dibenzo-p-dioxin Bioassays Dioxin Electrical Equipment In Vitro Study PCBs Polychlorinated Biphenyls TCDD
• ISSN:
  0272-0590
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  4
• Issue:
  6
• NIOSHTIC Number:
  nn:20060577
• Citation:
  Fundam Appl Toxicol 1984 Dec; 4(6):1036-1041
• Contact Point Address:
  Kandace J. Williams, Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire 03756 USA
• CAS Registry Number:
  2,3,7,8-Tetrachlorodibenzo-p-dioxin (CAS RN 1746-01-6)
• Federal Fiscal Year:
  1985
• Performing Organization:
  New York State Department of Health
• Peer Reviewed:
  True
• Start Date:
  19831101
• Source Full Name:
  Fundamental and Applied Toxicology
• End Date:
  19851231
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c1f4c5c3ebea306d909d051b904f3f69325af0ae102e5cfb11362df0af05fb7a985c0cf5fff5a1fb5a361e4d468840e1a7b39aeab22b49811d1161b807439774
• Download URL:
  https://stacks.cdc.gov/view/cdc/224440/cdc_224440_DS1.pdf
• File Type:
  [PDF - 432.23 KB ]