The Optimization of Methods for the Collection of Aerosolized Murine Norovirus

Details

• Personal Author:
  Boles C ; Brown G ; Nonnenmann M ; Park JH
• Description:
  Globally, norovirus is the most common gastroenteritis causing pathogen. Annually, norovirus causes 685 million cases of acute gastroenteritis and 200,000 deaths, worldwide. Recent evidence has suggested that norovirus can also be spread via aerosolization; however, an indoor generation source has yet to be determined. We optimized a sampling method for the collection of aerosolized norovirus using murine norovirus (MNV) as a surrogate. Optimization of the sampling method was performed using two bioaerosol samplers (SKC BioSampler and the NIOSH Bioaerosol Cyclone Sampler 251) and two sampling media (Hanks Balanced Salt Solution [HBSS] and Phosphate Buffered Saline [PBS]). Murine norovirus was aerosolized in a bioaerosol chamber and later collected using each sampler/media combination. Collected MNV was quantified using quantitative polymerase chain reaction (qPCR). Intact capsids of MNV were assessed using propidium monoazide dye in combination with qPCR and confirmed with transmission electron microscopy. Ten trials were conducted, with each trial lasting for 30 min. The SKC BioSampler collected a significantly higher concentration of MNV than the NIOSH-251 sampler did (p-value < 0.0001). However, there were no significant differences in the relative percent of MNV that remained viable between both samplers (p-value = 0.2215). The use of HBSS sampling media yielded a higher concentration of MNV than PBS media (p-value = 0.0125). However, PBS media maintained viability at a significantly higher percentage than HBSS media (p-value < 0.0001). The results support the optimization of a sampling method for the collection of aerosolized MNV and possibly norovirus in different sampling environments. [Description provided by NIOSH]
• Subjects:
  Aerosols Occupational Health Safety
• Keywords:
  Author Keywords: Aerosol Bioaerosol Bioaerosols Environmental Health Exposure Science Industrial Hygiene Norovirus Pathogens Samplers Sampling
• ISSN:
  1867-0334
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; Iowa ; Ohio ; OSHA Region 5 ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  199-208
• Volume:
  12
• Issue:
  3
• NIOSHTIC Number:
  nn:20059954
• Citation:
  Food Environ Virol 2020 Sep; 12(3):199-208
• Contact Point Address:
  Corey Boles, Cardno ChemRisk, Cincinnati, OH
• Email:
  corey.boles@cardno.com
• Federal Fiscal Year:
  2020
• Performing Organization:
  University of Iowa
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Food and Environmental Virology
• End Date:
  20290630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fd182d4fc11662f06f67a7e1664b4076de686806ab802043adadc8537545bb405f9b9521659f286603447d4535a8f22b9263344263db825b3233621a8d354d21
• Download URL:
  https://stacks.cdc.gov/view/cdc/224006/cdc_224006_DS1.pdf
• File Type:
  [PDF - 634.64 KB ]