Whole Genome Transcriptome Analysis in a Genetic Model of Gulf War Illness

Details

• Personal Author:
  Ashbrook D ; Jones BC ; Lu L ; Miller DB ; Mulligan MK ; O'Callaghan JP ; Williams RW ; Xu F ; Ashbrook D ; Jones BC ; Lu L ; Miller DB ; Mulligan MK ; O'Callaghan JP ; Williams RW ; Xu F
• Description:
  Gulf War illness (GWI) affected up to 30% of the nearly one million personnel sent to the Persian Gulf in 1991. The probable cause was exposure to organophosphorus compounds coupled with high circulating glucocorticoids as would be expected in a combat theater. Previously, we developed a mouse model consisting of 7 days of exposure to corticosterone in the drinking water followed by injection with diisopropylflurophosphate (DFP, surrogate for sarin) and assessment of pro-inflammatory cytokines in frontal cortex and hippocampus 6h after DFP treatment. In order to assess genetic-based individual differences in susceptibility to developing GWI, we applied the model to male and female C57BL/6J and DBA/2J mice. Consequently we observed wide-genetic differences in pro-inflammatory gene expression by qPCR in the prefrontal cortex. We then subjected the prefrontal cortex to genome-wide transcriptome response by RNA-seq, comparing the combined corticosterone- DFP treatment. Gene ontology analysis showed altered immune function and apoptosis as the top systems affected. We also verified a previous nomination of Spondin 1 and ILb1 as candidate genes underlying individual differences in susceptibility. [Description provided by NIOSH]
• Subjects:
  Adrenal Cortex Hormones Animals Genetics Immune System Laboratories Occupational Health Phosphoric Acids Safety Toxicology
• Keywords:
  Animal Studies Brain Function Brain Matter Cell Biology Cell Death Cellular Function Cellular Reactions Corticoids Corticosteroids Cytokines Exposure Assessment Gene Expression Genes Genetic Factors Genomics Immune Reaction Immune System Laboratory Animals Laboratory Testing Military Personnel Models Organophosphorus Compounds Phosphates Proteins Ribonucleic Acids RNA

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 9 ; Tennessee ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  174
• Issue:
  1
• NIOSHTIC Number:
  nn:20058989
• Citation:
  Toxicologist 2020 Mar; 174(1):488
• CAS Registry Number:
  (2,4-Dichlorophenoxy)acetic acid (CAS RN 94-75-7) ; (2,4-Dichlorophenoxy)acetic acid (CAS RN 94-75-7)
• Federal Fiscal Year:
  2020
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 59th Annual Meeting and ToxExpo, March 15-19, 2020, Anaheim, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1097600a6eb134b0303dd01d087f1b429de9e627c9900cc83bc062f04d9cc639ff70dc4f6e9e871ef4771ddba973464b425f8ea91e45c4b6f87098cdf63eadb5
• Download URL:
  https://stacks.cdc.gov/view/cdc/223329/cdc_223329_DS1.pdf
• File Type:
  [PDF - 521.90 KB ]