Biological Effects of Long-Term Exposure of Human BEAS-2B and MET-5A Cells to Riebeckite/Tremolite Asbestos and Their Respective Cleavage Fragments

Details

• Personal Author:
  Guppi S ; Harper M ; Khaliullin TO ; Kisin ER ; Shvedova AA ; Yanamala N
• Description:
  Asbestos is a commercial term that refers to 6 different fibrous minerals including riebeckite (RF) and tremolite (TF) amphibole asbestos. Inhalation of respirable forms of amphibole fibers, can cause asbestosis, lung cancer and both pleural and peritoneal mesothelioma. Amphiboles can also occur in a non-fibrous habit that can be mechanically broken into cleavage fragments (CF) which can meet the mineralogical/regulatory criteria for fibers. While the effects of RF and TF on health are well documented, there is uncertainty regarding the toxicity of riebeckite and tremolite CF. In this study, human epithelial (BEAS-2B) and mesothelial (MET-5A) cells were evaluated for the presence of several cancer hallmarks indicating the neoplastic-like transformation following continuous long-term (5 weeks) exposure to sub-toxic (2.5microg/cm2) concentrations of RF, TF and their CF. TF- and RF-exposed cells, both BEAS-2B and MET-5A, revealed a neoplastic-like transformation phenotype characterized by significant increase in invasion/migration, anchorage-independent growth, proliferation, and morphological transformation, compared to controls. No anchorage-independent growth and invasion was observed in both cell types treated with riebeckite CF although an increase in DNA damage, migration, proliferation, and morphological changes were detected in BEAS-2B cells. In the case of tremolite CF, although a significant increase in proliferation, transformation and DNA damage was observed in both cell types, an increase in invasion/migration and anchorage-independent growth was detected only in BEAS-2B cells. Similarly, analysis of inflammatory responses suggested cell-type specific effects as well as treatment related differences. Overall, our data are compatible with the findings that amphibole asbestos fibers demonstrate higher neoplastic transformation potential compared to the respective CF (at the same mass dose) in both bronchial epithelial and mesothelial cells. [Description provided by NIOSH]
• Subjects:
  Aluminum Compounds Aluminum Silicates Asbestos Cells, Cultured Immune System Inorganic Chemicals Minerals Neoplasms Occupational Health Particulate Matter Safety Toxicology

  More +
• Keywords:
  Aluminum Compounds Cancer Cell Cultures Cell Culture Techniques Cell Growth Cell Migration Cell Proliferation Cell Transformation Cell Type Cellular Reactions Cellular Structures DNA Damage Exposure Assessment Fibers Humans Immune Reaction Mesothelial Cells Morphology Neoplastic Transformation Nonmetallic Minerals Silicates

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; Florida ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  174
• Issue:
  1
• NIOSHTIC Number:
  nn:20058954
• Citation:
  Toxicologist 2020 Mar; 174(1):268
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4) ; Crocidolite asbestos (CAS RN 12001-28-4) ; Riebeckite (Fe5Na2H2(SiO3)8) (CAS RN 61105-30-4) ; Tremolite (Ca2[(Mg0.9-1Fe0-0.1)4.5-5Al0-0.5](Si7.5-8Al0-0.5)(OH)2O22) (CAS RN 14567-73-8)
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Mining
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 59th Annual Meeting and ToxExpo, March 15-19, 2020, Anaheim, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:04723bf8f9444f944d68f64c1294ce5237702a1506d0fb0c1acc5daa44c53c37839b85777743e4f5c332b8697492669277a4061d9dedcd38f9594469bc86325d
• Download URL:
  https://stacks.cdc.gov/view/cdc/223303/cdc_223303_DS1.pdf
• File Type:
  [PDF - 524.92 KB ]