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Enzyme-Linked Immunosorbent Assay Specific for (1–>6) Branched, (1–>3)-Beta-D-Glucan Detection in Environmental Samples



Details

  • Personal Author:
  • Description:
    (1-->3)-beta-d-Glucans have been recognized as a potential causative agent responsible for bioaerosol-induced respiratory symptoms observed in both indoor and occupational environments. A specific enzyme immunoassay was developed to quantify (1-->6) branched, (1-->3)-beta-d-glucans in environmental samples. The assay was based on the use of a high-affinity receptor (galactosyl ceramide) specific for (1-->3)-beta-d-glucans as a capture reagent and a monoclonal antibody specific for fungal cell wall B-d-glucans as a detector reagent. The assay was highly specific for (1-->6) branched, (1-->3)-beta-d-glucans (such as that from Saccharomyces cerevisiae) and did not show any response at 200 ng/ml to curdlan, laminarin, pustulan, dextran, mannan, carboxymethyl cellulose, and endotoxins. The detection level was 0.8 ng/ml for baker's yeast glucan and Betafectin. A coefficient of variation of 7.8% was obtained for (1-->3)-beta-d-glucans in house dust samples. Metal working fluids spiked with (1-->3)-beta-d-glucans inhibited the glucan assay. Because the assay is specific for (1-->6) branched, (1-->3)-beta-d-glucans and is sensitive and reproducible, it will be useful for the investigation of health effects from exposure to this class of biologically active molecules. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0099-2240
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Volume:
    67
  • Issue:
    12
  • NIOSHTIC Number:
    nn:20058798
  • Citation:
    Appl Environ Microbiol 2001 Dec; 67(12):5420-5424
  • Contact Point Address:
    Donald K. Milton, Occupational Health Program, Harvard School of Public Health, 665 Huntington Ave., Boston, MA 02115
  • Email:
    dmilton@hohp.harvard.edu
  • Federal Fiscal Year:
    2002
  • Performing Organization:
    Harvard University, Boston, Massachusetts
  • Peer Reviewed:
    True
  • Start Date:
    19980930
  • Source Full Name:
    Applied and Environmental Microbiology
  • End Date:
    20010929
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:0a97d24dbc7d8378bc493433b95815896d3bfec4bf32289a630f95fd790824d4246a9a32c6a5d0d222498f10d513313886607d36fc83f70015b818471e08cece
  • Download URL:
  • File Type:
    Filetype[PDF - 80.27 KB ]
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