A Clomazone Immunoassay to Study the Environmental Fate of the Herbicide in Rice (Oryza sativa) Agriculture

Details

• Personal Author:
  Cantou G ; Carlomagno M ; González D ; González-Sapienza G ; Hammock BD ; Last JA ; Mathó C ; Roel A ; Sanborn JR
• Description:
  The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzyme-linked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0-20 ng/mL range in field samples, with excellent correlation with high-performance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can contribute to minimizing the release of the herbicide in the environment. [Description provided by NIOSH]
• Subjects:
  Agriculture Environmental Monitoring Environmental Pollution Herbicides Insecticides Occupational Health Safety
• Keywords:
  Author Keywords: ELISA Clomazone Environmental Contamination Environmental Impacts Environmental Monitoring Ground Water HPLC Immunoassays Oryza Sativa Pesticides And Agricultural Chemicals Rice

  More +
• ISSN:
  0021-8561
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  58
• Issue:
  7
• NIOSHTIC Number:
  nn:20058727
• Citation:
  J Agric Food Chem 2010 Apr; 58(7):4367-4371
• Contact Point Address:
  Gualberto González-Sapienza, Cátedra de Immunología, Instituto de Higiene, DEPBIO, Facultad de Química, UdelarR, 11600 Montevideo, Uruguay
• Email:
  ggonzal@fq.edu.uy
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Agricultural and Food Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b3046d28762c95989df50fa9da50189b1bea75cd0be138404f043d2ff754dbda21f0c187975ff0c1e5d61284352fdb28cb5a52f48e1ecbd910e032357f4a1b93
• Download URL:
  https://stacks.cdc.gov/view/cdc/223128/cdc_223128_DS1.pdf
• File Type:
  [PDF - 880.96 KB ]