Mild Steel and Stainless Steel Welding Fumes Elicit Pro-Inflammatory and Pro-Oxidant Effects in First Trimester Trophoblast Cells

Details

• Personal Author:
  Bowers LN ; Friend SA ; Leonard, Stephen S. ; Morris AM ; Olgun, Nicole S. ; Reznik SE ; Stefaniak, Aleksandr B.
• Description:
  Problem: As more women join the skilled-trade workforce, the effects of workplace exposures on pregnancy need to be explored. This study aims to identify the effects of mild steel and stainless steel welding fume exposures on cultured placental trophoblast cells. Method of study: Welding fumes (mild steel and stainless steel) were generously donated by Lincoln Electric. Electron microscopy was used to characterize welding fume particle size and the ability of particles to enter extravillous trophoblast cells (HTR-8/SVneo). Cellular viability, free radical production, cytokine production, and ability of cells to maintain invasive properties were analyzed, respectively, by WST-1, electron paramagnetic resonance, DCFH-DA, V-plex MULTI-SPOT assay system, and a matrix gel invasion assay. Results: For all three welding fume types, average particle size was <210 nm. HTR-8/SVneo cells internalized welding particles, and nuclear condensation was observed. Cellular viability was significantly decreased at the high dose of 100ug/mL for all three welding fumes, and stainless steel generated the greatest production of the hydroxyl radical, and intracellular reactive oxygen species. Production of the cytokines IL-1B and TNFa were not observed in response to welding fume exposure, but IL-6 and IL-8 were. Finally, the invasive capability of cells was decreased upon exposure to both mild steel and stainless steel welding fumes. Conclusion: Welding fumes are cytotoxic to extravillous trophoblasts, as is evident by the production of free radicals, pro-inflammatory cytokines, and the observed decrease in invasive capabilities. [Description provided by NIOSH]
• Subjects:
  Free Radicals Immune System Metal Workers Occupational Health Particulate Matter Safety Welding
• Keywords:
  Author Keywords: Inflammation Cytokines Free Radicals Immune Reaction Metals Particulates Steel Industry Steelworkers Trophoblast Welding Welding Fumes

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• ISSN:
  1046-7408
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  83
• Issue:
  4
• NIOSHTIC Number:
  nn:20058541
• Citation:
  Am J Reprod Immunol 2020 Apr; 83(4):e13221
• Contact Point Address:
  Nicole S. Olgun, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  nolgun@cdc.gov
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Reproductive Immunology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:89b20bf725db4b701e6e5cc781eabad19a578b2136e7989054aa84e4f8c197118827dddbc14e0bef1681a1028b3d3b86527fc864b843dc999a4cd8d444382100
• Download URL:
  https://stacks.cdc.gov/view/cdc/221607/cdc_221607_DS1.pdf
• File Type:
  [PDF - 1.56 MB ]