Boric Acid Inhibits Adenosine Diphosphate-Ribosyl Cyclase Non-Competitively

Details

• Personal Author:
  Eckhert CD ; Faull KF ; Kim DH ; Norris AJ ; Que Hee S
• Description:
  Adenosine diphosphate-ribosyl cyclase (ADP-ribosyl cyclase) is a ubiquitous enzyme in eukaryotes that converts NAD+ to cyclic-ADP-ribose (cADPR) and nicotinamide. A quantitative assay for cADPR was developed using capillary electrophoresis to separate NAD+, cADPR, ADP-ribose, and ADP with UV detection (254 nm). Using this assay, the apparent Km and Vmax for Aplysia ADP-ribosyl cyclase were determined to be 1.24 +/- 0.05 mM and 131.8 +/- 2.0 uM/min, respectively. Boric acid inhibited ADP-ribosyl cyclase non-competitively with a Ki of 40.5 +/- 0.5 mM. Boric acid binding to cADPR, determined by electrospray ionization mass spectrometry, was characterized by an apparent binding constant, KA, of 655 +/- 99 L/mol at pH 10.3. [Description provided by NIOSH]
• Subjects:
  Chromatography Enzymes Occupational Health Safety
• Keywords:
  Adenosines Author Keywords: ADP-ribosyl Cyclase Boron Boron Oxide CADPR Capillary Electrophoresis Chromatographic Analysis Enzymatic Effects Enzyme Activity Enzyme Kinetics Mass Spectrometry NAD

  More +
• ISSN:
  0021-9673
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  246-252
• Volume:
  1115
• Issue:
  1
• NIOSHTIC Number:
  nn:20058366
• Citation:
  J Chromatogr A 2006 May; 1115(1-2):246-252
• Contact Point Address:
  Curtis D. Eckhert, Department of Environmental Health Sciences, Box 951772, University of California, 650 Charles E Young Dr South, Los Angeles, CA 90095-1772, USA
• Email:
  ceckhert@ucla.edu
• CAS Registry Number:
  Boric acid (H3BO3) (CAS RN 10043-35-3)
• Federal Fiscal Year:
  2006
• Performing Organization:
  University of California Los Angeles
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of Chromatography A
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5cdc2a22899ee79f16be5dea46d5d5af9a45bea8d68cb33e366a0344ff2e386a0bab6356c3075d01e4bc173abd1d00ac49c9b88329b63f5bbf9961913354236d
• Download URL:
  https://stacks.cdc.gov/view/cdc/221481/cdc_221481_DS1.pdf
• File Type:
  [PDF - 300.97 KB ]