Multiplex Sensor Platform for Biological Monitoring

Details

• Personal Author:
  Smith JN
• Description:
  This research project is focused on developing a multiplex sensor array for pesticides and their metabolites that are known to be readily excreted from blood to saliva. This novel platform will enable a more accurate prediction of occupational exposures, and once validated, can readily be employed to assess dosimetry of pesticide mixture exposures in support of biomonitoring and epidemiology studies. Three Specific Aims have been proposed: Aim 1. Develop a multiplex quantum-dot biosensor capable of detecting mixtures of pesticides, metabolites and cholinesterase activity at occupational concentrations. A rapid, sensitive, selective, and multiplex biosensor for detecting/screening of mixed pesticide exposures in readily obtainable biological fluids will be developed. The analytical performance (figure-of-merits) of this biosensor will be established, and the capability of the biosensor to simultaneously detect multiple biomarkers (e.g. parent pesticides, metabolites, ChE) will be validated in human samples (Aim 3). Aim 2. Estimate saliva and blood concentrations for occupational pesticide exposures utilizing a blood:saliva compartmental computational model. The goal is to utilize simple compartmental pharmacokinetic models for individual pesticides and metabolites to enable accurate prediction of saliva and blood analyte concentrations at relevant occupational exposure levels. This will directly support Aim 1 by establishing the target limits of detection (LOD) and quantification (LOQ). Aim 3. In vitro validation of biosensor for quantifying mixed pesticide exposures in human bio-matrices. Data will be used to validate the response of the multiplex biosensor (Aim 1) to quantify saliva, blood and urine pesticide concentrations following in vitro exposure to a mixture of pesticides over a range of relevant concentrations. [Description provided by NIOSH]
• Subjects:
  Agriculture Insecticides Occupational Exposure Occupational Health Safety
• Keywords:
  Biological Monitoring Biosensors Exposure Assessment Immunoassays Mixed Exposures Occupational Exposure Pesticides And Agricultural Chemicals Sensors
• Series:
  Grant Final Reports
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Final Grant Report
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OEP - Office of Extramural Programs
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-14
• NIOSHTIC Number:
  nn:20065167
• Citation:
  Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, R01-OH-011023, 2021 Dec; :1-14
• Contact Point Address:
  Jordan Ned Smith, BS, PhD, Battelle Pacific Northwest Laboratories, Box 999, 902 Battelle Blvd, Richland, WA 99352-0999
• Email:
  jordan.smith@pnnl.gov
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories, Richland, Washington
• Peer Reviewed:
  False
• Start Date:
  20160901
• Source Full Name:
  National Institute for Occupational Safety and Health
• End Date:
  20200831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2e07216950b796a89d7e691eea7822eea3713ef12d1fe6427d7a4887b0d492891709c3a443ee60162ade0fc746263de9dd2cbb92a6c78c369fc7d240169588be
• Download URL:
  https://stacks.cdc.gov/view/cdc/219109/cdc_219109_DS1.pdf
• File Type:
  [PDF - 272.68 KB ]