Asbestos Induced Alteration in Endothelial Cell Function

Details

• Personal Author:
  Treadwell MD
• Description:
  The purpose of this research was to investigate whether asbestos (1332214) induces an active endothelial cell phenotype, resulting in the increased expression of growth factors and proteases, which are relevant to the development of fibrosis. Porcine aortic endothelial cells were cultured with asbestos and ceramic fibers. The findings indicated that endothelial cells respond to noncytotoxic concentrations of asbestos fibers with alterations in specific cell functions, which may be relevant to pulmonary disease processes. The cells respond to both chrysotile (12001295) and crocidolite (12001284) asbestos fibers by changing cell morphology in focal areas of fiber contact, assuming a spindled appearance, indicative of the activated endothelial cell. A time dependent increase was noted in neutrophil binding to Intercellular-Adhesion-Molecule-1 and increases in both Urokinase-like-Plasminogen-Activator (uPA) mRNA levels and uPA activity. Endothelial cells exposed to chrysotile asbestos demonstrated in a time dependent manner an increase in the expression of the cell surface uPA receptor. Exposed cells also demonstrated an increase in the activation of the transcription activating factor Nuclear-Factor-kappa-B (NF-kB). Refractory-Ceramic- Fiber-Type-1 did not stimulate the endothelial cell morphology alteration, increase PMN binding, uPA mRNA or activity, or increase NF-kB nuclear translocation. [Description provided by NIOSH]
• Subjects:
  Asbestos Cells, Cultured Cytotoxins Dust Inorganic Chemicals Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Asbestos-fibers Cell-cultures Cytotoxic-effects Fibrous-dusts Lung-irritants Mammalian-cells NIOSH-Grant Pulmonary-system-disorders Respiratory-system-disorders
• Series:
  Grant Final Reports
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Final Grant Report
• Place as Subject:
  New Hampshire ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OEP - Office of Extramural Programs
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-6
• NIOSHTIC Number:
  nn:00238184
• NTIS Accession Number:
  PB97-206296
• Citation:
  The Environmental Affairs Group, Sanders A. Lockheed Martin Company, Nashua, New Hampshire 1997 Jan; :1-6
• Contact Point Address:
  Pharmacology and Toxicology Dartmouth Medical School Remsen 7650 Hanover, NH 03755-3835
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4) ; Chrysotile (CAS RN 12001-29-5) ; Crocidolite asbestos (CAS RN 12001-28-4)
• Federal Fiscal Year:
  1997
• NORA Priority Area:
  Pulmonary System Disorders
• Performing Organization:
  Dartmouth College, Hanover, New Hampshire
• Peer Reviewed:
  False
• Start Date:
  19940930
• Source Full Name:
  The Environmental Affairs Group, Sanders A. Lockheed Martin Company, Nashua, New Hampshire
• End Date:
  19960929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:92c99eb13bd29cc497d31fdd4d74f42e37128af380f25d7e9312eb3599dc979230ef1c2be892eca8b657c70bcff413d7f6adc9a2f032f57f6f1cf19e6d453f83
• Download URL:
  https://stacks.cdc.gov/view/cdc/217912/cdc_217912_DS1.pdf
• File Type:
  [PDF - 293.57 KB ]