Pathomechanisms of Chemically Induced Depigmentation

Details

• Personal Author:
  Gellin GA
• Description:
  High pressure liquid chromatographic analyses were made of skin from albino-mice and pigmented-mice, and melanoma cells (B16 transplantable hamster melanoma). Under the conditions of system-A, which detects dopa, cysteinyldopas and related compounds, the melanoma cells demonstrated six peaks. Pigmented ear skin showed four of these peaks and albino ear skin showed only one. Using system-B, which detects indole derivatives, two peaks were observed. Enzyme studies demonstrated that three glutathione metabolizing enzymes were elevated after exposure to 4-tertiary-butyl-catechol (98293) (TBC), an antioxidant. The food additive butylated- hydroxytoluene (128370) (BHT) was added to the food given to C57BL/6N-mice. Four weeks after feeding BHT the pigment was sparse and irregular. Electron microscopy studies were carried out on human skin from patients before and after treatment with PUVA. After 2 weeks of treatment many melanosomes demonstrated irregular deposition of pigment which was ultrastructurally interpreted as the start of pheomelanogenesis. Skin biopsy of a black woman with depigmentation who wore rubber gloves while working at a hotel showed no melanocytes. Tests revealed melanosomes with a pheomelanogenesis like ultrastructure. The author concludes that the basic methods needed to investigate chemically induced depigmentation have been developed. [Description provided by NIOSH]
• Subjects:
  Chromatography Laboratories Occupational Health Safety Skin Skin Diseases
• Keywords:
  Chromatographic-analysis Laboratory-animals NIOSH-Grant Pigmentation-disorders Skin-disorders Skin-exposure
• Series:
  Grant Final Reports
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Final Grant Report
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OEP - Office of Extramural Programs
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-7
• NIOSHTIC Number:
  nn:00181309
• NTIS Accession Number:
  PB88-247077
• Citation:
  Supplemental Information 1983 Jul; :1-7
• Contact Point Address:
  Dermatology University of California Department of Dermatology San Francisco, CA 94143
• CAS Registry Number:
  2,6-Di-tert-butyl-4-methylphenol (CAS RN 128-37-0) ; 4-tert-Butylcatechol (CAS RN 98-29-3)
• Federal Fiscal Year:
  1983
• Performing Organization:
  University of California San Francisco, San Francisco, California
• Peer Reviewed:
  False
• Start Date:
  19770701
• Source Full Name:
  Supplemental Information
• End Date:
  19890630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7a6fdc05eaab207c17679418051ba45a85baadfa73f9ffb702ad633da9ad60238b5ff918e9c942d6bb3ffd3bcc0da42f609491ea120b78457855117009e9b1ac
• Download URL:
  https://stacks.cdc.gov/view/cdc/217742/cdc_217742_DS1.pdf
• File Type:
  [PDF - 495.54 KB ]