Lack of Lung Tumor Promotion After Inhalation of a Copper-Nickel Welding Fume in A/J Mice

Details

• Personal Author:
  Andrews R ; Antonini J ; Battelli L ; Chen B ; Cumpston J ; Dodd T ; Donlin M ; Erdely A ; Falcone L ; Kashon M ; Keane M ; Leonard H ; McKinney W ; Mercer R ; Salmen R ; Stone S ; Zeidler-Erdely P
• Description:
  The International Agency for Research on Cancer classified welding fumes as a Group 1 carcinogen (carcinogenic to humans) in 2017. The process of stainless steel welding creates fumes rich in carcinogenic metals such as chromium (Cr). Our lab has previously demonstrated that stainless steel welding fumes promote lung tumors in tumor-susceptible A/J mice. Consumables devoid of Cr are being produced in an attempt to limit worker exposures to potentially carcinogenic metals. The aim of this study was to characterize a new copper-nickel (Cu-Ni) fume and then investigate if inhalation of this fume would promote lung tumors in mice using a two-stage (initiation-promotion) model. To determine particle mass size distribution, a Micro-Orifice Uniform Deposit Impactor (MOUDI, model 110; MSP corp., Shoreview, Minn.) with additional Nano-MOUDI stages (MSP model 115) was used. Characterization of the fume indicated that most of the particles were between 0.1 and 1 microm in diameter, with a mass median aerodynamic diameter of 0.43 microm. Male A/J mice (4 - 5 weeks old) were initiated with 3-methylcholanthrene (MCA; 10 microg/g IP) or corn oil and, beginning 1 week later, were exposed to air or Cu-Ni welding fumes for 4 hours/day, 4 days/week, for 9 weeks. At 30 weeks, mice were sacrificed and lung tumor multiplicity and incidence were evaluated. MCA/Cu-Ni welding fume exposure significantly decreased tumor number and tumor size compared to MCA/air controls (7.11 +/- 0.93 tumors vs. 15.57 +/- 0.75 tumors and 0.57 +/- 0.01 mm in diameter vs. 1.15 +/- 0.02 mm in diameter, respectively). Future studies are planned to investigate the pneumotoxicity of Cu-Ni fume in A/J mice. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Blood Carcinogens Cell Division Chromosomes Copper Environmental Exposure Hazardous Substances Laboratories Lung Metals Neoplasms Nickel Nucleotides Occupational Health Particulate Matter Respiratory System Safety Toxicology Welding

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• Keywords:
  Arc Welding Blood Cells Cell Culture Techniques Cell Division Cell Proliferation Cellular Effects Chromosome Damage Deoxyribonucleic Acids DNA DNA Damage Exposure Assessment Exposure Levels Inhalation Laboratory Animals Laboratory Testing Lung Tissue Particle Aerodynamics Particle Diameter Particle Size Particle Size Analysis Proteins Ribonucleic Acids RNA Signaling Pathways Stainless Steel Toxic Fumes Tumors Welding Fumes

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  168
• Issue:
  1
• NIOSHTIC Number:
  nn:20054947
• Citation:
  Toxicologist 2019 Mar; 168(1):240
• CAS Registry Number:
  3-Methylcholanthrene (CAS RN 56-49-5) ; Copper (CAS RN 7440-50-8) ; Nickel (CAS RN 7440-02-0) ; Stainless Steel (CAS RN 12597-68-1)
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 58th Annual Meeting and ToxExpo, March 10-14, 2019, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5bf62db8684ae9c023f1e3c718074b5ae81b9b3def0883e3da1f31b12eb4224f9247b8fbaf188b953a31ec3acabf514fe2d1a74081612e32d7520522f1d9e5ca
• Download URL:
  https://stacks.cdc.gov/view/cdc/217353/cdc_217353_DS1.pdf
• File Type:
  [PDF - 860.30 KB ]