Exposure to Traffic-Related Air Pollution, Home Dust, and the Respiratory Mycobiome

Details

• Personal Author:
  Cox J ; Meller J ; Uebel-Niemeier C
• Description:
  Background: Exposure to traffic-related air pollutants (TRAP) has been associated with exacerbation of existing asthma and incident asthma among young and adolescent children; Aggregates of elemental carbon nanoparticles are retained by the lung tissue, can induce pulmonary oxidative stress, and stimulate the inflammatory response by the release of cytokines from airway cells; TRAP can increase adherence of microorganisms to the epithelial cells of the respiratory tract by damaging cells in epithelial layers of respiratory tract; Bacterial diversity in sputum was found to be greater in asthmatic vs. non-asthmatic adult subjects; Similar to how the gut microflora affect the development of the immune system, the airway microbiome may interact with the immune system in the respiratory tract; Home health care workers are exposed to TRAP during travel between patients' homes. Indoor environment and work activities in patients' homes can contribute to elevated microbial exposures. Aims: Specific Aim 1: Characterize the fungal community profiles of the lower respiratory tract mycobiome. Specific Aim 2: Determine the association between exposure to TRAP and the mycobiome of the lower respiratory tract. Specific Aim 3: Characterize the fungal community profiles of home dust samples and compare the taxa and diversity indices to those of the lower respiratory mycobiomes of the two exposure groups. Methods: 40 participants ages 13-15 were recruited from the Cincinnati Childhood Allergy and Air Pollution study (CCAAPS) cohort from two a priori TRAP exposure categories: A. high exposure through age one and high exposure at early adolescence B. low exposure through age one and low exposure at early adolescence. Collected induced sputum, saliva, and home dust samples; Characterize fungal microbiome (mycobiome) of sputum and home dust samples using Illumina MiSeq with the amplification of the internal transcribed spacer (ITS) region of fungal ribosomal DNA; Measure total fungal DNA using quantitative PCR with universal fungal primers; Assess the associations between total fungal DNA, fungal operational taxonomic units (OTUs), and diversity indices of sputum and TRAP exposure; Compare the sputum mycobiome to the home dust mycobiome using principle component analysis of taxa and diversity indices, Expected Results: We hypothesize: that high exposure to TRAP alters the lower respiratory tract microbiome; that there are taxa that overlap between that fungal community and the home dust mycobiome. Future Directions: The knowledge gained from the proposed study provides preliminary data to conduct a larger investigation that focuses specifically on home health care workers and their occupational environments. Further research into respiratory health outcomes from these combined exposures. [Description provided by NIOSH]
• Subjects:
  Air Pollution Asthma Asthma, Occupational Dust Fungi Home Care Services Microbiology Occupational Health Respiratory System Safety
• Keywords:
  Air Pollution Children Dust Exposure Home Health Care Respiratory Function
• Publisher:
  University of Cincinnati
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1
• NIOSHTIC Number:
  nn:20054904
• Citation:
  19th Annual Pilot Research Project Symposium, University of Cincinnati Education and Research Center, October 11-12, 2018, Cincinnati, Ohio. Cincinnati, OH: University of Cincinnati, 2018 Oct; :1
• Email:
  uebelcm@mail.edu
• Federal Fiscal Year:
  2019
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  19th Annual Pilot Research Project Symposium, University of Cincinnati Education and Research Center, October 11-12, 2018, Cincinnati, Ohio
• End Date:
  20260630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8d9da2a479e5f6b88aaa83ae46917ab55da370326174dabea69bc88ff335b93f72942c8ddaff2e8f72c33d1ad09c54ca56d0298b9ee07db6a789b05155ceed3f
• Download URL:
  https://stacks.cdc.gov/view/cdc/217316/cdc_217316_DS1.pdf
• File Type:
  [PDF - 321.42 KB ]