(1 –> 3) Β-Glucan Induces Multimodal Toxicity Responses in Parallel Exposures of Model Human Lung Epithelial Cells and Immature Macrophage

Details

• Personal Author:
  Hernandez M ; McCabe K ; Snawder, John E. ; Turner J
• Description:
  Many epidemiological studies have associated bioaerosol exposures with a variety of adverse health effects; however, the role of bioaerosol components in the development and manifestation of hypersensitivity and non-infectious respiratory diseases remains unclear. Despite many studies which have examined allergic responses to bioaerosols, less is known about non-allergenic effects. In order to elucidate the mechanisms by which bioaerosols can exert non-atopic stresses on a cellular level, there is a need for improving existing in vitro approaches. In response, a cohort of toxicology assays were optimized to create a robust analytical suite for studying the effects that biogenic atmospheric pollutants generate on two model human lung cell lines (A549 epithelial line and GDM-1 immature macrophage line). To demonstrate the utility for studying the cellular responses to select bioaerosols, cells exposed to curdlan (a linear (1->3)-ß-glucan) were examined in a composite cytometry platform. Results suggest that curdlan has the potential to elicit significant responses in A549 and GDM-1 in two or more toxicological modes associated with exposure to airborne particulate matter. As designed, this suite provided a more powerful tool for characterizing curdlan-induced toxicological potential than any individual assay. Responses to curdlan were distinctly modal and cell line dependent, suggesting that the use of a suite of toxicological assays, in a common platform on different cell lines, can help provide important insights into the formative toxigenic responses that primary bioaerosols can induce in respiratory cells. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Blood Epidemiology Hazardous Substances Lung Macrophages Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Toxicology

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• Keywords:
  Airborne Particulates Author Keywords: Airborne Particulate Matter Bioaerosol Bioaerosols Cellular Reactions Curdlan Humans In Vitro In Vitro Studies In Vitro Study Lung Cells Particulates Pollutants Respiratory Diseases Toxicity

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• ISSN:
  1873-9318
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; Ohio ; OSHA Region 5 ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  379-387
• Volume:
  12
• Issue:
  4
• NIOSHTIC Number:
  nn:20054148
• Citation:
  Air Qual Atmos Health 2019 Apr; 12(4):379-387
• Contact Point Address:
  Hernandez, M., Civil, Environmental and Architectural Engineering, University of Colorado, Boulder, CO
• Email:
  mark.hernandez@colorado.edu
• Federal Fiscal Year:
  2019
• Peer Reviewed:
  True
• Source Full Name:
  Air Quality, Atmosphere & Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:43d9d84cc3823e37ce84963d1c61b4fbcebe840c2a17bc0037e0fcd452f8147360bed58eceb1b96e2a56ffa2635c6eb707e6ef43896f46349f266666462cde23
• Download URL:
  https://stacks.cdc.gov/view/cdc/216773/cdc_216773_DS1.pdf
• File Type:
  [PDF - 965.21 KB ]