Circulating miRs-183-5p, -206-3p and -381-3p May Serve as Novel Biomarkers for 4,4′-Methylene Diphenyl Diisocyanate Exposure

Details

• Personal Author:
  Hettick JM ; Law, Brandon F. ; Lin C-C ; Siegel, Paul D.
• Description:
  Background: Occupational exposure to the most widely used diisocyanate, 4,4'-methylene diphenyl diisocyanate (MDI), is a cause of occupational asthma (OA). Early recognition of MDI exposure and sensitization is essential for the prevention of MDI-OA. Objective: Identify circulating microRNAs (miRs) as novel biomarkers for early detection of MDI exposure and prevention of MDI-OA. Materials and methods: Female BALB/c mice were exposed to one of three exposure regimens: dermal exposure to 1% MDI in acetone; nose-only exposure to 4580 +/- 1497 ug/m3 MDI-aerosol for 60 minutes; or MDI dermal exposure/sensitization followed by MDI-aerosol inhalation challenge. Blood was collected and miRCURY(TM) miRs qPCR Profiling Service was used to profile circulate miRs from dermally exposed mice. Candidate miRs were identified and verified from mice exposed to three MDI-exposure regimens by TaqMan(R) miR assays. Results: Up/down-regulation patterns of circulating mmu-miRs-183-5p, -206-3p and -381-3p were identified and verified. Circulating mmu-miR-183-5p was upregulated whereas mmu-miRs-206-3p and -381-3p were downregulated in mice exposed via all three MDI exposure regimens. Discussion and conclusion: Upregulation of circulating miR-183-5p along with downregulation of circulating miRs-206-3p and -381-3p may serve as putative biomarkers of MDI exposure and may be considered as potential candidates for validation in exposed human worker populations. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Asthma, Occupational Biomarkers Isocyanates Occupational Health Respiratory System Safety Skin
• Keywords:
  4,4'-methylene Diphenyl Diisocyanate 4,4'methylenebisphenyl Isocyanate Animal Studies Author Keywords: Occupational Asthma Circulating MicroRNAs Dermal Exposures Diisocyanates DNCOs Inhalation Studies MDI MiRs OA Occupational Asthma

  More +
• ISSN:
  1354-750X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  76-90
• Volume:
  24
• Issue:
  1
• NIOSHTIC Number:
  nn:20054083
• Citation:
  Biomarkers 2019 Jan; 24(1):76-90
• Contact Point Address:
  Chen-Chung Lin, NIOSH/HELD/ACIB, 1095 Willowdale Rd., MS L4020, Morgantown, WV 26505, USA
• Email:
  mjy2@cdc.gov
• CAS Registry Number:
  Diphenylmethane diisocyanate (CAS RN 101-68-8)
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Biomarkers
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f155357d8efc3ffa4ff3f25bfb60d10f0056e66419344fca74f440ff286cfa877d55993a637be911518c273fcfe2a105f0a1491c126667b4071ce3cc0ce39c28
• Download URL:
  https://stacks.cdc.gov/view/cdc/216720/cdc_216720_DS1.pdf
• File Type:
  [PDF - 2.41 MB ]