Metabolism of the Antibacterial Triclocarban by Human Epidermal Keratinocytes to Yield Protein Adducts

Details

• Personal Author:
  Buchholz BA ; Hammock BD ; Rice RH ; Schebb NH
• Description:
  Previous studies of triclocarban suggest that its biotransformation could yield reactive metabolites that form protein adducts. Since the skin is the major route of triclocarban exposure, present work examined this possibility in cultured human keratinocytes. The results provide evidence for considerable biotransformation and protein adduct formation when cytochrome P450 activity is induced in the cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin, a model Ah receptor ligand. Since detecting low adduct levels in cells and tissues is difficult, we utilized the novel approach of accelerator mass spectrometry for this purpose. Exploiting the sensitivity of the method, we demonstrated that a substantial portion of triclocarban forms adducts with keratinocyte protein under the P450 inducing conditions employed. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Occupational Health Safety Skin
• Keywords:
  Antibacterial Agents Author Keywords: Accelerator Mass Spectrometry Biotransformation Cellular Effects Cytochrome P450 Keratinocytes Mass Spectrometry Metabolites Protein Adducts Protein Biochemistry Reactive Metabolites Skin Exposure TCDD Triclocarban

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• ISSN:
  1095-6670
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  230-234
• Volume:
  26
• Issue:
  6
• NIOSHTIC Number:
  nn:20054015
• Citation:
  J Biochem Mol Toxicol 2012 Jun; 26(6):230-234
• Contact Point Address:
  Robert H. Rice, Department of Environmental Toxicology, University of California, Davis, CA 95616, USA
• Email:
  rhrice@ucdavis.edu
• CAS Registry Number:
  Triclocarban (CAS RN 101-20-2)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  False
• Start Date:
  20010930
• Source Full Name:
  Journal of Biochemical and Molecular Toxicology
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:954f1bb60f0e6f066c38330374cfaa31abdb81a28f607e3a9d71c29dd65c7a4b0205534cf226484d60fcd2d011e44a6822814d2c2245f119cbacc6a77a9284f1
• Download URL:
  https://stacks.cdc.gov/view/cdc/216665/cdc_216665_DS1.pdf
• File Type:
  [PDF - 295.25 KB ]