Development of On-Line Liquid Chromatography-Biochemical Detection for Soluble Epoxide Hydrolase Inhibitors in Mixtures

Details

• Personal Author:
  Falck D ; Hammock BD ; Heus F ; Kool J ; Morriseau C ; Niessen WMA ; Prihatiningtyas S ; Schebb NH ; Zhang J
• Description:
  In this study, an end-point-based fluorescence assay for soluble epoxide hydrolase (sEH) was transformed into an on-line continuous-flow format. The on-line biochemical detection system (BCD) was coupled on-line to liquid chromatography (LC) to allow mixture analysis. The on-line BCD was based on a flow system wherein sEH activity was detected by competition of analytes with the substrate hydrolysis. The reaction product was measured by fluorescence detection. In parallel to the BCD data, UV and MS data were obtained through post-column splitting of the LC effluent. The buffer system and reagent concentrations were optimized resulting in a stable on-line BCD with a good assay window and good sensitivity (S/N > 60). The potency of known sEH inhibitors (sEHis) obtained by LC-BCD correlates well with published values. The LC-BCD system was applied to test how oxidative microsomal metabolism affects the potency of three sEHis. After incubation with pig liver microsomes, several metabolites of sEHis were characterized by MS, while their individual potencies were measured by BCD. For all compounds tested, active metabolites were observed. The developed method allows for the first time the detection of sEHis in mixtures providing new opportunities in the development of drug candidates. [Description provided by NIOSH]
• Subjects:
  Biological Assay Chromatography Energy Metabolism Enzymes Laboratories Occupational Health Safety
• Keywords:
  Author Keywords: Column Liquid Chromatography Bioassays Chromatographic Analysis Detectors Enzyme Activity Enzyme Inhibition Laboratory Techniques Metabolism Metabolites On-line Screening Soluble Epoxide Hydrolase

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• ISSN:
  0009-5893
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  13-21
• Volume:
  76
• Issue:
  1
• NIOSHTIC Number:
  nn:20054013
• Citation:
  Chromatographia 2013 Jan; 76(1-2):13-21
• Email:
  w.m.a.niessen@vu.nl
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Chromatographia
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8de9a82df15a2afd1aa25ce2770ee9b2e9dd5293fb5a4b3a600b3b73e73fd4aa345d5825a5d507171089516031e16a7de8f1fba6f2d693d3ae6f561c794c43a0
• Download URL:
  https://stacks.cdc.gov/view/cdc/216664/cdc_216664_DS1.pdf
• File Type:
  [PDF - 574.10 KB ]