Cell-Based High-Content Analysis (HCA) Reveals Differential Effects of BPA and Its Selected Analogues on Spermatogonia Stem Cells

Details

• Personal Author:
  Liang S ; Yin L ; Yu X
• Description:
  Bisphenol A (BPA), an endocrine-disrupting compound, is found to be a testicular toxicant in animal models. Bisphenol AF (BPAF), Bisphenol S (BPS) and Tetrabromobisphenol A (TBBPA), structurally similar to BPA, are introduced to the market as alternatives to BPA. However, there is limited toxicological data on the male reproductive systems of these compounds so far. In this study, we compared the time- and dose-dependent effects of BPA and its analogues on the cell viability and cell cycle alteration in spermatogonia stem cells. We observed dose-dependent decreases of cell viability using neutral red uptake assay at 24 and 48 h after treatments. The IC50 values at 48 h were 55.5, 58.5, 184.5 and 357.1 uM for TBBPA, BPAF, BPA and BPS, respectively. We established a high-throughput and high-content analysis (HCA) for the morphology and cell cycle analysis. The Hoechest 33342 staining was used to determine the nuclear size and nuclear morphology. 5-bromo-2'-deoxyuridine (BrdU) was used to label DNA synthesis for the active proliferating cells. Automated multi-channel images were acquired using ArrayScan VTI and quantified by HCS Studio 2. We found that BBAF and TBBPA significantly induced changes of nuclear shape at lower concentrations as compared to BPA and BPS, indicating a drastic condensation of chromatin. BBAF and TBBPA also significantly decreased the BrdU incorporation at non-toxicity doses at 72 h after treatments. In summary, our current study demonstrated the differential toxicity of BPA and its analogues on the spermatogonia cells. [Description provided by NIOSH]
• Subjects:
  Endocrine System Occupational Health Safety Spermatogenesis Toxicology Urogenital System
• Keywords:
  Cellular Effects Dose Response Endocrine Disruptors Reproductive Effects Toxic Effects
• ISSN:
  1096-6080
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Georgia ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  150
• Issue:
  1
• NIOSHTIC Number:
  nn:20053965
• Citation:
  Toxicologist 2016 Mar; 150(1):528
• CAS Registry Number:
  2,2-Bis(4-hydroxyphenyl)propane (CAS RN 80-05-7)
• Federal Fiscal Year:
  2016
• Performing Organization:
  University of Georgia, Athens
• Peer Reviewed:
  False
• Start Date:
  20130901
• Source Full Name:
  The Toxicologist. Society of Toxicology 55th Annual Meeting and ToxExpo, March 13-17, 2016, New Orleans, Louisiana
• End Date:
  20160831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:78fa0b42bc20b06022bfd255fe05597e468da03dad03b45a4557a3aef871e156e1c02ad3ed05f828cff994108c2ddcfe082ce6683e987a2aa53e035c62f2869a
• Download URL:
  https://stacks.cdc.gov/view/cdc/216621/cdc_216621_DS1.pdf
• File Type:
  [PDF - 498.76 KB ]