Preparation of Rodent Testis Co-Cultures

Details

• Personal Author:
  Faustman EM ; Hong S ; Wegner S ; Yu X
• Description:
  Male reproductive development is a complex process that is sensitive to disruption by a range of toxicants. There is a great need for in vitro models that can evaluate potential male reproductive toxicants. The current unit presents a protocol for preparation of a three-dimensional in vitro model of male reproductive development that reduces the number of animals required for evaluation of toxicants. A Matrigel overlay provides a three-dimensional extracellular matrix that improves cell attachment, viability, and communication, and makes the model more reflective of in vivo environments. [Description provided by NIOSH]
• Subjects:
  Laboratories Occupational Health Safety Spermatogenesis Urogenital System
• Keywords:
  Author Keywords: Male Reproductive Development Cellular Effects Laboratory Animals Primary Cell Culture Reproductive Effects Testes Three-dimensional In Vitro Models Toxic Effects
• ISSN:
  1934-9262
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; OSHA Region 10 ; OSHA Region 4 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  55
• Issue:
  1
• NIOSHTIC Number:
  nn:20053964
• Citation:
  Curr Protoc Toxicol 2013 Feb; 55(1):16.10.1-16.10.7
• Federal Fiscal Year:
  2013
• Performing Organization:
  University of Georgia, Athens
• Peer Reviewed:
  False
• Start Date:
  20130901
• Source Full Name:
  Current Protocols in Toxicology
• End Date:
  20160831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9627e2b871d67efce96d962d59671ba92a3f747ca1faa8484e60e679f1e87a9598a29eb8b9cf229748ce747cec0a40776f6dd64a4f46e4641bf9c618ec5bcc87
• Download URL:
  https://stacks.cdc.gov/view/cdc/216620/cdc_216620_DS1.pdf
• File Type:
  [PDF - 195.64 KB ]