Organic Dust, Lipopolysaccharide, and Peptidoglycan Inhalant Exposures Result in Bone Loss/Disease

Details

• Personal Author:
  Bauer C ; Duryee MJ ; Dusad A ; Gleason AM ; Klassen LW ; Mikuls TR ; Poole JA ; Romberger DJ ; Thiele GM ; West WW
• Description:
  Skeletal health consequences associated with chronic inflammatory respiratory disease, and particularly chronic obstructive pulmonary disease (COPD), contribute to overall disease morbidity. Agricultural environmental exposures induce significant airway diseases, including COPD. However, animal models to understand inhalant exposure-induced lung injury and bone disease have not been described. Using micro-computed tomography (micro-CT) imaging technology and histology, bone quantity and quality measurements were investigated in mice after repetitive intranasal inhalation exposures to complex organic dust extracts (ODEs) from swine confinement facilities. Comparison experiments with LPS and peptidoglycan (PGN) alone were also performed. After 3 weeks of repetitive ODE inhalation exposure, significant loss of bone mineral density and trabecular bone volume fraction was evident, with altered morphological microarchitecture changes in the trabecular bone, compared with saline-treated control animals. Torsional resistance was also significantly reduced. Compared with saline treatment, ODE-treated mice demonstrated decreased collagen and proteoglycan content in their articular cartilage, according to histopathology. Significant bone deterioration was also evident after repetitive intranasal inhalant treatment with LPS and PGN. These findings were not secondary to animal distress, and not entirely dependent on the degree of induced lung parenchymal inflammation. Repetitive LPS treatment demonstrated the most pronounced changes in bone parameters, and PGN treatment resulted in the greatest lung parenchymal inflammatory changes. Collectively, repetitive inhalation exposures to noninfectious inflammatory agents such as complex organic dust, LPS, and PGN resulted in bone loss. This animal model may contribute to efforts toward understanding the mechanisms and evaluating the therapeutics associated with adverse skeletal health consequences after subchronic airway injury. [Description provided by NIOSH]
• Subjects:
  Dust Farmers Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Lung Bioaerosol Bioaerosols Bone Bone Disorders Bone Structure Chronic Obstructive Pulmonary Disease COPD Dust Exposure Farm Workers Imaging Inflammation Lung Disease Respirable Dust Respiratory Diseases

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• ISSN:
  1044-1549
• Document Type:
  Text
• Funding:
  Grant ; Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  49
• Issue:
  5
• NIOSHTIC Number:
  nn:20053154
• Citation:
  Am J Respir Cell Mol Biol 2013 Nov; 49(5):829-838
• Contact Point Address:
  Jill A. Poole, M.D., Pulmonary, Critical Care, Sleep, and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, 985300 The Nebraska Medical Center, Omaha, NE 68198-5300
• Email:
  japoole@unmc.edu
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  American Journal of Respiratory Cell and Molecular Biology
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d72967cf90d78f01ad7d99a3bfe5dc6b0ff89b557aafc63a7968dc026fdf0334582d1fe5caf67b2e2ae07ceeaa5c74711041312a753a62b3f49d3f6f0162cb6f
• Download URL:
  https://stacks.cdc.gov/view/cdc/215968/cdc_215968_DS1.pdf
• File Type:
  [PDF - 2.63 MB ]