Effect of Elevated Carbon Dioxide on Innate Immune Response in the Lung

Details

• Personal Author:
  Bailey KL ; Cloonan D ; Devasure JM ; Romberger D ; Schneberger D ; Wyatt T
• Description:
  Purpose/Objective: Workers in high intensity livestock facilities exhibit reduced lung function over time in the industry. Many symptoms have been attributed to barn dust exposure and bacterial components in these dusts that can activate innate immune responses. Under circumstances of decreased ventilation of barn facilities and increased pulmonary obstruction, the lungs of these workers may also be exposed to elevated levels of carbon dioxide (CO2). Methods/Efforts: In recent years there have been conflicting reports on the effects of CO2 on inflammation in the lung from studies of permissive hypercapnia. We proposed these changes would be reflected in lung epithelial cells. To address this we looked at several cell lines representing bronchial epithelium, alveolar epithelium, and alveolar macrophages and how they responded to stimulation with a variety of innate immune stimuli as well as barn dust under normal (5%) and elevated (9%) CO2. Results/Findings: We show that all cell types tested showed an increase in production of inflammatory signaling molecules in response to higher CO2, suggesting that CO2 may enhance inflammation in the lung, or make these cells more responsive to potential inflammatory molecules in the environment. Interestingly, expression of anti-microbial molecules produced by these same cells showed reductions in several at elevated CO2, suggesting that killing of microbes may at the same time be inhibited by CO2. In fact barn dust exposure itself may be inhibiting this one microbial defense mechanism. Application to Field Research: The study of hypercapnia and its effect on innate immunity is a basic study that has immediate implications for work on smoking, workplace safety, ventilator lung strategy, and infection in cases of chronic obstruction. [Description provided by NIOSH]
• Subjects:
  Agriculture Carbon Dioxide Dust Immune System Livestock Lung Occupational Health Respiratory System Safety
• Keywords:
  Agricultural Safety Biological Effects Carbon Dioxide Cellular Effects Dust Exposure Humans Immune Reaction Livestock Industry Lung Function
• Publisher:
  International Society for Agricultural Safety and Health (ISASH)
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  Iowa ; Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1
• NIOSHTIC Number:
  nn:20052874
• Citation:
  2014 International Society for Agricultural Safety and Health (ISASH) Annual Conference, June 23-25, 2014, Omaha, Nebraska. Urbana, IL: International Society for Agricultural Safety and Health (ISASH), 2014 Jun; :1
• Email:
  d.schneberger@unmc.edu
• CAS Registry Number:
  Carbon dioxide (CAS RN 124-38-9)
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Iowa, Iowa City
• Peer Reviewed:
  False
• Start Date:
  20010930
• Source Full Name:
  2014 International Society for Agricultural Safety and Health (ISASH) Annual Conference, June 23-25, 2014, Omaha, Nebraska
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:380901abff15994f0ca00f186b3df4e184f5cc27b0413b548f1985878adc830061a5ebcca893d6df031dd56e2f55c8688f8e9eb2778d23b914403732bce34b45
• Download URL:
  https://stacks.cdc.gov/view/cdc/215786/cdc_215786_DS1.pdf
• File Type:
  [PDF - 188.44 KB ]