A Comparison of Techniques for Preparing Fish Fillet for ICP-AES Multielemental Analysis and the Microwave Digestion of Whole Fish

Details

• Personal Author:
  Ambrose RF ; Moeller A ; Que Hee SS
• Description:
  Four catfish fillet homogenate treatments before multielemental metal analysis by simultaneous inductively coupled plasma/atomic emission spectroscopy were compared in triplicate. These treatments were: nitric acid wet-ashing by Parr bomb digestion; nitric acid wet-ashing by microwave digestion; tetramethylammonium hydroxide/nitric acid wet digestion; and dryashing. The tetramethylammonium hydroxide/nitric acid method was imprecise (coefficients of variation > 20%;). The dry-ashing method was fast and sensitive but had low recoveries of 50% for spiked Pb and Al and was not as precise as the Parr bomb or microwave treatments. The Parr bomb method was the most precise method but was less sensitive than the microwave method which had nearly the same precision. The microwave method was then adapted to homogenates of small whole fish ≤3cm in length. The whole fish homogenate required more vigorous digestion conditions, and addition of more acid after the evaporative step because of the presence of less oxidizable and acidsoluble components than fillet. The whole fish homogenate was also more heterogeneous than catfish fillet. A quality assurance protocol to demonstrate homogenate uniformity is essential. The use of a non-specialized microwave oven system allowed precise results for fillet and whole fish homogenates. [Description provided by NIOSH]
• Subjects:
  Laboratories Metals Occupational Health Safety Spectrum Analysis
• Keywords:
  Author Keywords: Fish Elements Environmental Health Monitoring Fish Digestion Food Contaminants ICP Analysis Laboratory Techniques Metallic Compounds Microwave Digestion Multielemental Analysis Spectrographic Analysis
• ISSN:
  0265-203X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  19-29
• Volume:
  18
• Issue:
  1
• NIOSHTIC Number:
  nn:20057904
• Citation:
  Food Addit Contam 2001 Jan; 18(1):19-29
• Contact Point Address:
  Shane S. Que Hee, Department of Environmental Health Sciences and UCLA Center for Occupational and Environmental Health, UCLA School of Public Health, 10833 Le Conte Avenue, Los Angeles, CA 90095- 1772, USA
• Email:
  squehee@ucla.edu
• Federal Fiscal Year:
  2001
• Performing Organization:
  University of California, School of Public Health, Los Angeles, CA
• Peer Reviewed:
  True
• Start Date:
  19990701
• Source Full Name:
  Food Additives and Contaminants
• End Date:
  20040630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a76a20cda336d88ca6a339ad70ca33ec6ce95907a2476228d8e391a66055daed026f354f907c279454611d2e8ef16a3ad32d8543b15298b2e7971a48df727674
• Download URL:
  https://stacks.cdc.gov/view/cdc/215108/cdc_215108_DS1.pdf
• File Type:
  [PDF - 214.36 KB ]