Analysis of HIV-1 Protease Gene Reveals Frequent Multiple Infections Followed by Recombination among Drug Treated Individuals Living in São Paulo and Santos, Brazil

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Nunes, Edsel Renata De Morais ; Zukurov, Jean Paulo ; Maricato, Juliana Terzi ; Sucupira, Maria Cecília Araripe ; Diaz, Ricardo Sobhie ; Janini, Luíz Mário Ramos
• Description:
  The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil.Detection of multiple infections was done using a previously published RFLP assay for the HIV-1 protease gene, which is able of distinguishing between infections caused by a single or multiple HIV-1 subtypes. Samples with multiple infections were cloned, and sequence data submitted to phylogenetic analysis. We were able to identify 17 HIV-1 multiple infections out of 47 samples. Multiple infections were mostly composed by a mixture of recombinant viruses (94%), with only one case in which protease gene pure subtypes B and F were recovered. This is the first study that reports the prevalence of multiple infections and intersubtype recombinants in a population undergoing HAART in Brazil. Based on the data there was a steep increase of multiple infections after the introduction of the combined antiretroviral therapy in Brazil. Cases of multiple infections may be associated with HIV-1 genetic diversity through recombination allowing for the generation of viruses showing a combination of resistance mutations.
• Subjects:
  Biology Medicine Research Article
• Source:
  PLoS One. 2014; 9(1).
• Document Type:
  Journal Article
• Volume:
  9
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:0dada119d6eb838612d019eb29d988006e285e81dfe33891fa73f0a5c1bbf5ef
• Download URL:
  https://stacks.cdc.gov/view/cdc/21503/cdc_21503_DS1.pdf
• File Type:
  [PDF - 1.13 MB ]