Details:

Alternative Title:
PLoS One
Personal Author:
Nunes, Edsel Renata De Morais ; Zukurov, Jean Paulo ; Maricato, Juliana Terzi ; Sucupira, Maria Cecília Araripe ; Diaz, Ricardo Sobhie ; ... More +
Nunes, Edsel Renata De Morais ; Zukurov, Jean Paulo ; Maricato, Juliana Terzi ; Sucupira, Maria Cecília Araripe ; Diaz, Ricardo Sobhie ; Janini, Luíz Mário Ramos ; Less -
Description:
The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil.Detection of multiple infections was done using a previously published RFLP assay for the HIV-1 protease gene, which is able of distinguishing between infections caused by a single or multiple HIV-1 subtypes. Samples with multiple infections were cloned, and sequence data submitted to phylogenetic analysis. We were able to identify 17 HIV-1 multiple infections out of 47 samples. Multiple infections were mostly composed by a mixture of recombinant viruses (94%), with only one case in which protease gene pure subtypes B and F were recovered. This is the first study that reports the prevalence of multiple infections and intersubtype recombinants in a population undergoing HAART in Brazil. Based on the data there was a steep increase of multiple infections after the introduction of the combined antiretroviral therapy in Brazil. Cases of multiple infections may be associated with HIV-1 genetic diversity through recombination allowing for the generation of viruses showing a combination of resistance mutations.
Subject:
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Biology
Medicine
Research Article

Source:
PLoS One. 2014 ; 9(1).;
Document Type:
Journal Article ;
Collection(s):
CDC Public Access
Main Document Checksum:
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urn:sha256:0dada119d6eb838612d019eb29d988006e285e81dfe33891fa73f0a5c1bbf5ef
File Type:

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