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Metabolite Formation and Toxicity Measurements in Evaluating Bioremediation of a Jet-Fuel-Contaminated Aquifer



Details

  • Personal Author:
  • Description:
    The metabolic capabilities of subsurface, jet-fuel-contaminated, aquifer microbial communities were characterized using an ecological approach to biotreatment assessment. A multifaceted experimental design was used that incorporated quantification of metabolite formation and toxicity screening along with the typical microbial activity measurements and pollutant degradation measurements used for assessing bioremediation potential. For select experiments, dissolved oxygen levels and pH in microcosm systems were also monitored. Results suggest that a sizable, metabolically active microbial community exists in both contaminated and uncontaminated areas of the study site. Time course metabolism analyses indicated that the microbial communities were capable of degrading all three test compounds (amino acids, decane, and toluene) without any apparent adaptation period. Measurements of mineralization, cellular uptake, and metabolite formation indicated that metabolite formation was the predominant fate of the target pollutants in the microcosms. The results of toxicity screening time courses indicated that under oxygen-limiting conditions, the potential for the accumulation of toxic, acidic metabolites that could adversely affect the rates and extent of bioremediation existed. The experimental results indicate that the microbial communities at the site possess the metabolic potential for in situ biodegradation of the jet fuel. Care must be taken in the design of an in situ biodegradation treatment system (for this site and perhaps other petroleum-contaminated aquifers) to avoid the development of microaerophilic or oxygen-depleted zones; which could result in possible self-poisoning owing to acidic metabolite accumulation. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0273-2289
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Pages in Document:
    79-97
  • Volume:
    76
  • Issue:
    2
  • NIOSHTIC Number:
    nn:20057716
  • Citation:
    Appl Biochem Biotechnol 1999 Feb; 76(2):79-97
  • Contact Point Address:
    Sharon C. Long, Department of Civil and Environmental Engineering, University of Massachusetts, 18 Marston Hall, Amherst, MA, 01003
  • Email:
    long@ecs.umass.edu
  • Federal Fiscal Year:
    1999
  • Performing Organization:
    Arnold School of Public Health, University of South Carolina, Columbia
  • Peer Reviewed:
    True
  • Start Date:
    19940701
  • Source Full Name:
    Applied Biochemistry and Biotechnology
  • End Date:
    20040630
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:92915fb2a47bc1100b4def4c57b012d17abf0e9716075ea823045cfdcae100c3c086c81080e2976b25fe8efed871e4e8a74621c6d826fc71332562fd0c6fa5b7
  • Download URL:
  • File Type:
    Filetype[PDF - 185.36 KB ]
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