Identification of Novel Breast Tumor-Specific Mutation(s) in the q11.2 Region of Chromosome 17 by RAPD/AP-PCR Fingerprinting

Details

• Personal Author:
  Roy D ; Singh KP
• Description:
  Analysis of genetic instability in breast cancer tissues compared to uninvolved breast tissues from the same individuals by RAPD (random amplified polymorphic DNA)/AP-PCR (arbitrarily primed PCR) fingerprinting using 30 arbitrary primers revealed 190 amplified DNA fragments. Presumably, each of these represents a gene locus in a different region of the genome of breast cancer tissues. Among these amplified DNA fragments, 65 (34.2%) exhibited presence and absence or reductions and enhancements in the intensity in breast cancer tissues compared to uninvolved breast tissues from the same individuals, and 11 amplified DNA fragments (5.7%) represented polymorphisms in the uninvolved human breast tissues. Reductions and enhancements in the intensity of some of the amplified fragments were observed indicating allelic gains or losses in the breast tumor genome compared to the matched uninvolved tissue genome. The presence or absence of some of the amplified DNA fragments were observed in this study indicating homozygous deletions or insertions in the breast tumor DNA compared to the matched uninvolved tissue DNA. Notably, an insertion of a 1270 bp amplified fragment was observed in 81% (17 of 21) of the tumor samples using the primer, OPC04. This amplified fragment resolved into two, 1200 and 1300 bp, single-stranded amplified fragments on the denaturing sequencing gel. This separation into single-stranded fragments suggests that the amplified fragment contains a conformation that is semistable. The 1270 bp amplified fragment localizes to the q11.2 region of chromosome 17. Sequence analysis of this fragment showed a significant DNA base sequence similarity (93%) with one of the breast tumor-specific human EST. The similarity with EST sequences and RT-PCR analysis showed that a part of this amplified fragment is from the coding region of the genome. Any one of the events observed in this study could play an important role in the development of breast cancer or could occur during the clonal expansion of the genetically unstable breast cells. [Description provided by NIOSH]
• Subjects:
  Chromosomes Occupational Health Safety
• Keywords:
  Amplification Author Keywords: Arbitrary Primer Breast Cancer Chromosome Damage DNA Damage Genetic Factors Genetic Instability Humans Polymorphism
• ISSN:
  0378-1119
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Alabama ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  33-43
• Volume:
  269
• Issue:
  1
• NIOSHTIC Number:
  nn:20057621
• Citation:
  Gene 2001 May; 269(1-2):33-43
• Contact Point Address:
  Deodutta Roy, Department of Environmental Health Sciences, 1665 University Blvd., Ryals Bldg. #309E, University of Alabama at Birmingham, Birmingham, AL 35294-0022, USA
• Email:
  Royd@uab.edu
• Federal Fiscal Year:
  2001
• Performing Organization:
  Deep South Center for Occupational Health and Safety, University of Alabama at Birmingham, Birmingham, AL
• Peer Reviewed:
  True
• Start Date:
  19980701
• Source Full Name:
  Gene
• End Date:
  20040630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:366b0ace0b8365b261553eb5ece43c957aaed40d1fcf6290b8307b4009b00e1a62ccc531f26a96f5ac279805704227d9d245c4c057c26f7aedf0bf20e63babd7
• Download URL:
  https://stacks.cdc.gov/view/cdc/214894/cdc_214894_DS1.pdf
• File Type:
  [PDF - 1.05 MB ]